Open Access Highly Accessed Research article

Development, screening, and analysis of DNA aptamer libraries potentially useful for diagnosis and passive immunity of arboviruses

John G Bruno1*, Maria P Carrillo1, Alicia M Richarte1, Taylor Phillips1, Carrie Andrews1 and John S Lee2

Author Affiliations

1 Operational Technologies Corporation, 4100 NW Loop 410, Suite 230, San Antonio, TX, 78229, USA

2 U.S. Army Medical Research and Materiel Command, Military Infectious Disease Research Program, 504 Scott Street, Bldg. 722, Fort Detrick, MD, 21702, USA

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BMC Research Notes 2012, 5:633  doi:10.1186/1756-0500-5-633

Published: 13 November 2012

Additional files

Additional file 1:

Comprehensive anti-arbovirus aptamer sequence list.

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Additional file 2:

Figure S1. Secondary structures of the top ten ELASA ranked Chikungunya aptamers. Figure S2. Secondary structures of the top ten ELASA ranked Crimean-Congo Hemorrhagic Fever (CCHF) Altamura Gn611 aptamers. Figure S3. Secondary structures of the top ten ELASA ranked CCHF 11E7a aptamers. Figure S4. Secondary structures of the top ten ELASA ranked CCHF 11E7b aptamers. Figure S5. Secondary structures of the top ten ELASA ranked CCHF 11E7c aptamers. Figure S6. Secondary structures of the top ten ELASA ranked CCHF Drosdov strain whole virus-developed aptamers. Figure S7. Secondary structures of consensus CCHF IbAr 10200 strain whole virus-developed aptamers in the entire aptamer library. Note that the reverse sequence contains the most common ACGGGTCCGGACA sequence segment (underlined) in its structure as well. Figure S8. Secondary structures of the top ten ELASA ranked dengue serotype 1 aptamers. Figure S9. Secondary structures of the top ten ELASA ranked dengue serotype 2 aptamers. Figure S10. Secondary structures of the top ten ELASA ranked dengue serotype 3 aptamers. Figure S11. Secondary structures of the top ten ELASA ranked dengue serotype 4 aptamers. Figure S12. Secondary structures of the top ten ELASA ranked Tick-borne Encephalitis Virus (TBEV) aptamers. Figure S13. Secondary structures of the top ten ELASA ranked West Nile Virus (WNV) aptamers.

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