Workflow overview. Eight plasma samples from healthy volunteers were collected into a pool to obtain four technical replicates. After cut-off, each replicate was processed by SPE on a robotic device containing three different resins: anionic, cationic and liphophilic. The 24 obtained samples were further fractioned by nanoHPLC-MALDI-TOF-TOF analysis.
Boccardi et al. BMC Research Notes 2012 5:612 doi:10.1186/1756-0500-5-612