Quantitative differences in miRNA expression in medulloblastoma (MB) normalising to different EC reference genes. The mean fold changes of hsa-miR-923, hsa-miR-144* and hsa-miR-21* expression in nine primary MB specimens in comparison to the mean expression of these miRNAs in CD133+ NSCs and CD133- NPCs. Relative expression was determined using the 2-ΔCq method and log2 transformed. Normalisation was carried out either to hsa-miR-425* (dark blue), RNU43 (red), hsa-miR-877 (green), RNU24 (purple) or geometric mean of hsa-miR-425* and RNU24 (light blue). The fold changes of all three miRNAs normalised against RNU43 was different from the fold changes obtained following the normalisation against hsa-miR-425*, hsa-miR-877, RNU24 and the geometric mean of hsa-miR-425* and RNU24.
Genovesi et al. BMC Research Notes 2012 5:507 doi:10.1186/1756-0500-5-507