|Post sequencing analysis of raw reads|
|Sample||Treatment||Flowcell lane||Number of reads||Q.S. (median)||Q.S. (interquartile range)|
For each sample, the total number of reads and read quality score (QS) is listed. A QS of 34 indicates one sequencing error per 4,000 base pairs. Generally, a QS over 20 (1% error rate) is considered acceptable for RNA-Seq. One control (−) and one elevated ozone (+) replicate were pooled and run on a single lane of the flow-cell.
Yendrek et al.
Yendrek et al. BMC Research Notes 2012 5:506 doi:10.1186/1756-0500-5-506