Table 1

Post sequencing analysis of raw reads
Sample Treatment Flowcell lane Number of reads Q.S. (median) Q.S. (interquartile range)
1 Ambient 4 36,408,402 34 26-36
2 Elevated O3 4 28,554,551 34 26-36
3 Ambient 5 16,862,414 34 29-37
4 Elevated O3 5 17,575,844 34 29-37
5 Ambient 6 31,889,531 34 28-37
6 Elevated O3 6 37,605,167 34 28-37

For each sample, the total number of reads and read quality score (QS) is listed. A QS of 34 indicates one sequencing error per 4,000 base pairs. Generally, a QS over 20 (1% error rate) is considered acceptable for RNA-Seq. One control (−) and one elevated ozone (+) replicate were pooled and run on a single lane of the flow-cell.

Yendrek et al.

Yendrek et al. BMC Research Notes 2012 5:506   doi:10.1186/1756-0500-5-506

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