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Open Access Research article

Plasma profile of microRNA after supplementation with high doses of vitamin D3 for 12 months

Rolf Jorde1*, Johan Svartberg1, Ragnar Martin Joakimsen1 and Dag H Coucheron2

Author Affiliations

1 Tromsø Endocrine Research Group, Department of Clinical Medicine, University of Tromsø, Tromsø, Norway and Division of Internal Medicine, University Hospital of North Norway, 9038, Tromsø, Norway

2 RNA and Transcriptomics Group, Department of Medical Biology, University of Tromsø, Tromsø, Norway

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BMC Research Notes 2012, 5:245  doi:10.1186/1756-0500-5-245

Published: 17 May 2012

Abstract

Background

Recently a large number of short non-coding-RNAs (microRNAs, (miRNA)) have been identified. These miRNAs act as post-transcriptional regulators where they generally have an inhibitory function. miRNAs are present in all human cells, and they are also detected in serum or plasma. The miRNAs have a broad range of actions, and their biogenesis must therefore be under tight control. One putative regulator of miRNA biogenesis or miRNA level could be vitamin D, an ancient hormone with effects on cell growth and differentiation, apoptosis and the immune system. In our study miRNA were reversed transcribed in total RNA isolated from plasma and analyzed by quantitative real-time PCR (qPCR) using the miRCURY LNA Universal RT microRNA PCR system (Exiqon). In 10 pilot subjects 136 miRNAs were detected in one or more plasma samples drawn at baseline and after 12 months of vitamin D supplementation. The twelve miRNAs that showed the greatest change in expression in these pilots were further analyzed by RT-qPCR of RNA from baseline and 12 months plasma samples in 40 subjects given high dose vitamin D3 (20.000 – 40.000 IU per week) and 37 subjects given placebo.

Results

At baseline there was a significant and positive correlation between serum 25-hydroxyvitamin D and miR-532-3p expression (r = 0.24, P = 0.04). The change in expression of miR-221 from baseline to 12 months (ddCp value) was also significantly different between the vitamin D and placebo group (P =0.04), mainly due to a change in the placebo group.

Conclusions

We have not been able to demonstrate a consistent effect of vitamin D supplementation on the expression profile of miRNA in plasma. However, further studies are needed as this approach might potentially throw light on unknown aspects of vitamin D physiology.

Keywords:
microRNA; Randomized clinical trial; Vitamin D