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Open Access Research article

MYOD-1 in normal colonic mucosa – role as a putative biomarker?

Ramesh P Arasaradnam12*, M Nabil Quraishi1, Daniel Commane3, John C Mathers3 and Mike Bradburn4

Author affiliations

1 Department of Gastroenterology, University Hospital Coventry & Warwickshire, Coventry, UK

2 Clinical Sciences Research Institute, Warwick Medical School, Clifford Bridge Road, Coventry, CV2 2DX, UK

3 Human Nutrition Research Institute, Newcastle University, Newcastle, UK

4 Department of Colorectal Surgery, Wansbeck General Hospital, Wansbeck, Northumberland, UK

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Citation and License

BMC Research Notes 2012, 5:240  doi:10.1186/1756-0500-5-240

Published: 16 May 2012

Abstract

Background

DNA methylation of promoter-associated CpG islands of certain genes may play a role in the development of colorectal cancer. The MYOD-1 gene which is a muscle differentiation gene has been showed to be significantly methylated in colorectal cancer which, is an age related event. However the role of this gene in the colonic mucosa is not understood and whether methylation occurs in subjects without colon cancer. In this study, we have determined the frequency of methylation of the MYOD-1 gene in normal colonic mucosa and investigated to see if this is associated with established colorectal cancer risk factors primarily ageing.

Results

We analysed colonic mucosal biopsies in 218 normal individuals and demonstrated that in most individuals promoter hypermethylation was not quantified for MYOD-1. However, promoter hypermethylation increased significantly with age (p < 0.001 using regression analysis) and this was gender independent. We also showed that gene promoter methylation increased positively with an increase in waist to hip (WHR) ratio – the latter is also a known risk factor for colon cancer development.

Conclusions

Our study suggests that promoter gene hypermethylation of the MYOD-1 gene increases significantly with age in normal individuals and thus may offer potential as a putative biomarker for colorectal cancer.

Keywords:
MYOD-1; Colorectal cancer; Age; Promoter methylation