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Akt and phospholipase Cγ are involved in the regulation of growth and migration of MDA-MB-468 breast cancer and SW480 colon cancer cells when cultured with diabetogenic levels of glucose and insulin

Nicola M Tomas1*, Kai Masur12, Jonas C Piecha1, Bernd Niggemann1 and Kurt S Zänker1

Author Affiliations

1 Institute of Immunology and Experimental Oncology, Witten/Herdecke University, Stockumer Str. 10, D-58448, Witten, Germany

2 Institute for Plasma Research and Technology e.V. – INP Greifswald, Felix-Hausdorff-Str. 2, D-17489, Greifswald, Germany

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BMC Research Notes 2012, 5:214  doi:10.1186/1756-0500-5-214

Published: 3 May 2012

Abstract

Background

Epidemiological studies revealed a strong correlation between the metabolic syndrome/diabetes mellitus type 2 (DM2) and higher incidence and faster progression of breast and colon cancer. However, the underlying molecular mechanisms are widely unknown. Akt and phospholipase Cγ (PLCγ) are involved in tyrosine kinase signaling and promote tumor cell growth and migration. Therefore, we examined regulatory functions and expression of Akt and PLCγ in a simplified in vitro diabetogenic model.

Findings

Protein expression was determined by western blot analysis in MDA-MB-468 breast cancer and SW480 colon cancer cells previously cultured under physiologic (5.5 mM) and diabetogenic (11 mM) glucose concentrations (without and with 100 ng/ml insulin). We studied the culture effects on proliferation and migration of these cells, especially after inhibiting Akt and PLCγ. We found that Akt expression was up-regulated with high glucose and insulin in both cell lines, whereas PLCγ expression was enhanced in colon cancer cells only. High levels of glucose and insulin increased cell proliferation and migration in both cell lines in vitro, mediated by Akt and PLCγ, as shown through the specific pharmacological inhibitors A6730 and U73122.

Conclusions

Our molecular data explain glucose- and insulin-induced changes in a cancer cell and help to understand what might trigger tumor cell proliferation and migration in DM2 patients, too.

Keywords:
Breast and colon cancer; Diabetes mellitus type 2; Glucose; Insulin; Akt; PLCγ