Flow diagram of the study design: Peripheral blood was drawn from 3 healthy young male volunteers at 12:00 and 3:00 pm using PAXgene™ and Tempus™ collection tubes. After the first blood draw at baseline, volunteers were administered 1.5 mg dexamethasone orally and blood was collected again after 3 h. After blood collection, RNA (24 samples, 8 from each individual) was extracted using standard protocols and later processed either with or without globin reduction. Blood from 2 PAXgene™ tubes was pooled together to obtain enough RNA for the amplifications and the globin reduction procedure.
Menke et al. BMC Research Notes 2012 5:1 doi:10.1186/1756-0500-5-1