Table 1

Primer pair sequences and allele distribution of 10 microsatellites among clone A, clone D and clone A⁺ of gibel carp*

Locus (YJ)

Primer pair sequence

Annealing tempreture

Allele size (bp)

Clone A

Clone D

Clone A⁺

0001

5'-CTGGCATGAAGACTGGCTC-3'

5'-CAACAACACATATCAGCTCC-3'

53°C

84, 94, 100

88, 92, 96

84, 94, 100

0003

5'-TGAAGTTATTAGAAAGAGAG-3'

5'-CTTGATGATGTCTATGTGTG-3'

53°C

270, 288, 302

260, 290, 292

270, 288, 302

0004

5'-CATAGAGGCGTTTCATAGAG-3'

5'-CAGATAAATACAGTAAGCCA-3'

50 - 55°C

210, 220, 224

218, 222, 226

210, 220, 224

0005

5'-TAATAAGGTACATAGTCATAG-3'

5'-GTCAGCCTCCACCACGAATC-3'

50 - 55°C

226, 230, 234

222

226, 230, 234

0033

5'-CGGACACAAGAACGCCAAC-3'

5'-GGACTGGGCTGAAACTGATG-3'

50 - 55°C

172, 178

176

172, 178

0039

5'-GAAGAATACTTTATGACTGAGG-3'

5'-GACCAAGACAGACAGCCCAG-3'

50 - 55°C

138, 150, 156

136, 138, 156

138, 150, 156

0042

5'-GGCCACCTACAGTATATGC-3'

5'-GAAAACCAGGACCGACATG-3'

50 - 55°C

110, 114, 116

110, 112, 118

110, 114, 116

0040

5'-CCAGTATTAGGGAGCGTTC-3'

5'-GTTTCGTCTTCACAATCAGAA-3'

50 - 55°C

124, 138

132, 148

124, 138

0010

5'-GATGGTTGTGCTGTGAGCT-3'

5'-GAGTTCGTTTACATCTGGAC-3'

53°C

150, 156, 166

152, 160, 162

150, 156, 166

0022

5'-CACCAACTTTAGGCACATTTG-3'

5'-CCAGACTCCCACGTCATG-3'

53°C

144, 156

140, 162, 170

144, 156

Total

10

27

25

27

*For each clone and each generation, 10 individuals were sampled and analyzed, and clone A⁺ samples of three generations, such as the third, fifth and seventh generation, were examined. No any individual difference was detected within each clone and among different generations.

Wang et al. BMC Research Notes 2011 4:82   doi:10.1186/1756-0500-4-82