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Open Access Highly Accessed Technical Note

MPT 64 Antigen detection for Rapid confirmation of M.tuberculosis isolates

Vijay GS Kumar1*, Tejashree A Urs2 and Rajani R Ranganath3

Author Affiliations

1 Professor & Head of Clinical Microbiology, JSS Medical College, Mysore, 570 015, India

2 Professor of Microbiology, JSS Medical College, Mysore, 570 015, India

3 Post graduate student, Department of Microbiology, JSS Medical College, Mysore, 570 015, India

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BMC Research Notes 2011, 4:79  doi:10.1186/1756-0500-4-79

Published: 24 March 2011

Abstract

Background

A new rapid Immunochromatographic test kit(SD MPT64TB Ag Kit) for detection of MPT 64 Antigen in M. tuberculosis isolates using mouse monoclonal MPT 64 Antibody developed by SD Bioline, South Korea was evaluated for rapid identification of M. tuberculosis isolates. We also assessed the sensitivity, specificity and predictive values of this kit. The test kit has an excellent sensitivity, specificity, negative predictive value & positive predictive value. This rapid method is found to be a reliable, rapid and cheaper method for confirming MTB culture isolates in resource poor laboratories. Material/methods: 54 culture isolates of M. tuberculosis in broth & on LJ medium, 12 Non mycobacterial isolates, 10 Non tubercular (NTM) rapidly growing Mycobacteria isolated from pus & 5 smear positive sputum samples were tested for detection of MPT64 antigen using the SD Bioline immunochromatography (ICT)test kit. H37 RV strain was employed as the positive reference control.

Findings

H37 RV strain showed the presence of MPT64 antigen band. Similar band was formed in all the 54 MTB isolates tested proving 100% sensitivity. MPT64 band formation was not detected in any of the other test isolates which proved the 100% specificity of the test kit. Both PPV & NPV were 100%.

Conclusion

Tuberculosis is a global pandemic. Rapid identification of MTB culture isolate is very important for drug susceptibility testing. MPT 64 TB Ag detection ICT kit is a rapid, reliable method; it can be a substitute for the molecular identification methods.