Table 3

Individual identification through genotyping: Six microsatellite loci (PUN124, PUN 229, PUN935, PUN1157, PUN132 and PUN894) were selected for individual identification.

SITE

YEAR

IND.

PUN124

PUN229

PUN935

PUN1157

PUN132

PUN894


Shey Phoksundo National Park

Shey

2008

1

90

96

106

112

121

121

101/

103

103

118

118

110

110

Shey

2008

2

96

90

102

106

115

119

105

105

112

116

110

110

Shey

2008

2

96

90

102

106

115

119

105

105

112

116

110

110

Shey

2008

3

90

92

106

112

121

121

101

101

118

120

110

110

Dho

2008

4

94

96

102

106

119

121

101

105

118

118

110

110

Kanchenjunga Conservation Area

Yagma

2009

5

96

98

106

110

115

119

103

105

112

118

110

110

Gunsa

2009

6

89

98

106

110

119

123

103

105

112

118

110

118

Yagma

2009

7

-

-

110

112

115

115/

119

101/

109

109

118

118

110

118

Gunsa

2009

8

96

96

108/

110

110

115/

119

119

105/

101

105

112

112

110

110

Gunsa

2006

9

96

96

110

110

115

119

101

105

112

118

110

110


Ten samples, which were snow leopard positive on species PCR identification, successfully amplified across all 6 microsatellite loci. There were two samples from Shey area of SPNP that had complete match across all 6 microsatellite loci and were identified as samples from the same individual. A total of 9 snow leopards were identified from SPNP and KCA.

Karmacharya et al. BMC Research Notes 2011 4:516   doi:10.1186/1756-0500-4-516

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