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Open Access Research article

Antiviral mode of action of bovine dialyzable leukocyte extract against human immunodeficiency virus type 1 infection

Humberto H Lara*, Liliana Ixtepan-Turrent, Elsa N Garza-Treviño, Jose I Badillo-Almaraz and Cristina Rodriguez-Padilla

Author Affiliations

Laboratorio de Inmunología y Virología, Departamento de Microbiología e Inmunología, Universidad Autonoma de Nuevo Leon, Nuevo Leon, Mexico

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BMC Research Notes 2011, 4:474  doi:10.1186/1756-0500-4-474


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1756-0500/4/474


Received:24 July 2011
Accepted:1 November 2011
Published:1 November 2011

© 2011 Lara et al; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Bovine dialyzable leukocyte extract (bDLE) is derived from immune leukocytes obtained from bovine spleen. DLE has demonstrated to reduce transcription of Human Immunodeficiency Virus Type 1 (HIV-1) and inactivate the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway. Therefore, we decided to clarify the mode of antiviral action of bDLE on the inhibition of HIV-1 infection through a panel of antiviral assays.

Results

The cytotoxicity, HIV-1 inhibition activity, residual infectivity of bDLE in HIV-1, time of addition experiments, fusion inhibition of bDLE for fusogenic cells and the duration of cell protection even after the removal of bDLE were all assessed in order to discover more about the mode of the antiviral action.

HIV-1 infectivity was inhibited by bDLE at doses that were not cytotoxic for HeLa-CD4-LTR-β-gal cells. Pretreatment of HIV-1 with bDLE did not decrease the infectivity of these viral particles. Cell-based fusion assays helped to determine if bDLE could inhibit fusion of Env cells against CD4 cells by membrane fusion and this cell-based fusion was inhibited only when CD4 cells were treated with bDLE. Infection was inhibited in 80% compared with the positive (without EDL) at all viral life cycle stages in the time of addition experiments when bDLE was added at different time points. Finally, a cell-protection assay against HIV-1 infection by bDLE was performed after treating host cells with bDLE for 30 minutes and then removing them from treatment. From 0 to 7 hours after the bDLE was completely removed from the extracellular compartment, HIV-1 was then added to the host cells. The bDLE was found to protect the cells from HIV-1 infection, an effect that was retained for several hours.

Conclusions

bDLE acted as an antiviral compound and prevented host cell infection by HIV-1 at all viral life cycle stages. These cell protection effects lingered for hours after the bDLE was removed. Interestingly, bDLE inhibited fusion of fusogenic cells by acting only on CD4 cells. bDLE had no virucidal effect, but could retain its antiviral effect on target cells after it was removed from the extracellular compartment, protecting the cells from infection for hours.

bDLE, which has no reported side effects or toxicity in clinical trials, should therefore be further studied to determine its potential use as a therapeutic agent in HIV-1 infection therapy, in combination with known antiretrovirals.

Background

The pandemic of Human Immunodeficiency Virus Type 1 (HIV-1) infection, the cause of Acquired Immunodeficiency Syndrome (AIDS), is a grave public health issue and ranks among the greatest infectious disease scourges in history [1]. There were more than 33.3 million people worldwide with HIV-1 infection or AIDS, according to the latest estimates by the Joint United Nations Program on HIV/AIDS (UNAIDS) [2].

The use of highly active antiretroviral therapies has dramatically reduced morbidity and mortality among patients infected with HIV-1 [3,4]. However, the success of antiretroviral treatment is frequently restricted by the emergence of HIV-1 drug resistance [5]. Therefore, the search for new drugs to inhibit viral replication [6] or to restore the immune system in HIV-1 patients continues. Newly discovered naturally derived or chemically synthesized substances are continuously being evaluated as therapeutic drug candidates with antiviral activity. These potential drugs are eagerly awaited and may prove beneficial for the growing number of HIV-infected individuals who have developed resistance to the currently available antiretrovirals [7].

Dialyzable Leukocyte Extract (DLE) is derived from immune leukocytes and contains low molecular weight proteins (< 10, 000 Da) [8]. DLE possess three chromatographic fractions (Fa, Fb and Fc), Fraction Fb inhibits viral production more than 80%. Therefore, fractions Fa and Fc did not show inhibitory effect for any viral dose used [9].

This preparation is a modulator of the immune response that is able to transmit the ability to express delayed-type hypersensitivity (DTH) and cell mediated immunity (CMI) from sensitized donors to immune deficient recipients [10]. DLE also mediates effects on immune system functions, further influencing its response. These effects include cytokine modulation [11,12], the activation of monocyte and macrophage chemotaxis [13] and natural killer activity enhancement [14]. The therapeutic and prophylactic applications have been the most important and interesting aspects of DLE [15], principally because there has been no reported side effects or toxicity in humans [16].

DLE has demonstrated to be effective in those diseases in which Cell-Mediated Immunity (CMI) plays a relevant role in protection against and control of the disease, such as viral infections ((herpes zoster [17], hepatitis B [18], intracellular bacterial diseases like tuberculosis [19] and leprosy [20], parasite infections, such as leishmaniasis [21] or cryptosporidiosis [22], and fungal infections (mucous-cutaneous candidiasis [23]), as well as in primary immunodeficiencies (Wiskott Aldrich syndrome [24], Behçet's syndrome [25]), bronchial asthma [26], otitis media [27], uveitis [28]) and some types of cancer [29,30].

Previously we reported that bovine DLE (bDLE) was useful as an adjuvant in breast cancer patients undergoing chemotherapy, demonstrating protective effects against myelosuppression secondary to antitumoral drugs by improving cellular and humoral immunity, as well as in regulating the production of different cytokines involved in cellular proliferation [16,29,30]. Furthermore, in vitro assays demonstrated that bDLE affected the regulation of the expression of p53, bab-1, c-myc, bax, bcl-2 and bad mRNA [31,32]. Nowadays, the majority of the studies on DLE are limited to diseases that occur with chronic inflammation [33], like HIV-1 infection.

A main feature of HIV infection is the expression of several proinflammatory cytokines expressed as soluble factors or membrane-bound molecules that regulate both HIV replication and T cell apoptosis. Proinflammatory cytokines have key roles in the HIV lifecycle, especially at the level of transcription, by enhancing the ability of HIV to establish latent reservoirs on HIV infected patients. In addition, several HIV proteins, such as Nef, Tat, and Vpr hijack proinflammatory cytokine signaling, further underlining the potential importance of inflammation in HIV pathogenesis. Moreover, an in vivo chronic inflammatory state has been correlated to increased levels of viremia and accelerated disease progression [34]. DLE has been used to treat HIV-1 infected patients, either asymptomatic or at the AIDS phase, resulting in a partial immune reconstitution [35,36], a lower incidence of opportunistic infections [37], and clinically relevant improvement [16,38].

Viruses have evolved to modulate the NF-kB pathway to enhance viral replication, improve host cell survival, and evade the immune response [39]. With HIV, viral and cellular membrane fusion activates NF-κB, a process that requires CD4+ T cells. HIV-1 contains regulatory regions in its long terminal repeat (LTR) implicated in the control of viral gene expression that contain three Sp1 core promoter binding sites and two NF-κB core enhancer motifs [40] that are recognized by endogenous host cell transcription factors. These are important regulatory elements in the LTR that control expression of the promoter along with Tat, a viral transactivator protein necessary for HIV-1 replication [41].

Previous studies have reported DLE in vitro reduced HIV-1 transcription [42] by regulating activation of NF-κB and Sp1 transcription factors [42-44]. Other studies reported DLE induced the production of leukocytes and reduced TNF-α [44] and TFG-β1 [45] secretion, which are cytokines that play a pivotal role in HIV-1 pathogenesis by up-regulating the transcription of HIV-1 and increasing the expression of HIV co-receptor CXCR4, respectively. Additionally, envelope glycoprotein gp120 can signal NF-κB by engaging the CD receptor in a pathway that involves p56 and activates NF-κB and HIV-1 LTR transcription [46]. HIV-1 gene expression and transcription is an essential step in the viral life cycle and is considered to be a possible target for the inhibition of HIV-1 replication [43]. The exact mechanism of action of bDLE is still unclear, however we focused on the bDLE mode of action against HIV-1 infection.

Results

Cytotoxic effect

The half cytotoxic concentration (CC50) of bDLE when exposed to HeLa-CD4-LTR-β-gal cells was 3.41 ± 0.1 IU (P < 0.0001) (Figure 1A).

thumbnailFigure 1. Cytotoxicity assessment of bDLE and HIV-1 inhibition activity. A) HeLa-CD4-LTR-β-gal cells (5 × 104 cells/well) and B) HIV-1IIIB cell-free viruses (MOI 0.2-0.5) were challenged with two-fold serial dilutions of bDLE. Cell viability and β-gal activity were measured with a luciferase-based assay 24 h after nanosilver exposure. Percentage values are relative to the positive control (no compound treatment). The data represent the means ± standard deviations from three separate experiments, each of which was carried out in duplicate.

Range of antiviral activity

bDLE was tested against an HIV-1IIIB isolate using indicator cells in which infection was quantified by a luciferase-based assay. The concentration of bDLE at which HIV-1IIIB infectivity was inhibited by 50% (IC50) was found to be 1.53 ± 0.1 IU (P < 0.0001) (Figure 1B). In addition, bDLE inhibited HIV-1IIIB infectivity at doses that were not cytotoxic for HeLa-CD4-LTR-β-gal cells. The therapeutic index (TI = CC50/IC50) for bDLE in these cells was then calculated to be 2.23. The therapeutic index reflects a compound's overall efficacy by relating cytotoxicity (CC50) with effectiveness, measured as the ability to inhibit infection (IC50), under the same assay conditions.

Virucidal activity

To determine if the bDLE might have effects on the virus itself, HIV-1IIIB isolates were treated with different concentrations of bDLE. After removal of bDLE, the residual infectivity of the cell-free viruses was quantified by a luciferase-based assay. As shown in Figure 2, bDLE pretreatment of HIV-1IIIB did not decrease the infectivity of the viral particles in a dose dependent manner.

thumbnailFigure 2. Residual activity in HIV-1 strains. HIV-1IIIB cell-free viruses were exposed to serial dilutions of bDLE for 5 minutes. The viruses were then ultracentrifuged, washed twice and added to HeLa-CD4-LTR-β-gal cells. After 24 hours, β-gal activity was measured. Percentage values are relative to the positive control (infected cells without bDLE treatment). The data represent the means ± standard deviations from three separate experiments, each of which was carried out in duplicate.

Inhibition of Env/CD4-mediated membrane fusion

A cell-based fusion assay was used to mimic the gp120-CD4-mediated fusion process of HIV-1 with bDLE. When bDLE was exposed first to the CD4 cells-Env cells mixture, fusion between both cells was blocked in a dose-dependent manner. Cell-based fusion was also inhibited when bDLE was applied only to CD4 cells for 30 minutes, followed by bDLE removal. However, when Env cells were first exposed to bDLE for 30 minutes, then removed and added to CD4 cells, fusion between both cells was not inhibited (Figure 3A). Known antiretroviral drugs, such as UC781 (NNRTI), were used as controls in this cell based fusion assay and did not inhibit cell fusion (Figure 3B). T-20 (Fusion Inhibitor), did inhibit cell fusion in all assays, however, except when exposed to CD4 cells for only 30 minutes and then removed, after which the cells were mixed (Figure 3C).

thumbnailFigure 3. Inhibition of Env/CD4-mediated membrane fusion. β-gal activity was measured after CD4 cells and Env cells were co-cultured after exposure to A) bDLE, B) UC781 and C) T-20 under different circumstances: (■) CD4 cells were exposed to the compound and co-cultured with Env cells for 24 hours; (▲) CD4 cells were exposed to the compound for 30 minutes, washed, and co-cultured with Env cells for 24 hours; (✘) Env cells were exposed to the compound for 30 minutes, washed, and co-cultured with CD4 cells for 24 hours. Percentage values are relative to the positive control (cell-to-cell fusion without pretreatment with drug). The data represent the means ± standard deviations from three separate experiments, each of which was carried out in duplicate.

Time (Site) of Intervention

To further determine the antiviral target of bDLE, a time-of-addition experiment was performed using a single cycle infection assay. The time-of-addition experiment was used to determine the stage(s) of the viral life cycle that were blocked by bDLE. Several antiretroviral drugs were chosen as controls as they mark different stages of the viral cycle (i.e., fusion or entry, retrotranscription, protease activity, and integration into the genome). As seen in Figure 4(A-E), the antiviral activity of T-20, UC781, 118-D-24 and Amprenavir started to decline after the cycle stage that they targeted was passed. The fusion inhibitor's activity declined after 2 h (Figure 4B), the RT inhibitor after 8 h (Figure 4C), the integrase inhibitor after 18 h (Figure 4D) and the protease inhibitor after 15-18 h (Figure 4E). In contrast, bDLE retained its antiviral activity up to 48 h (Figure 4A) after the HIV inoculation, inhibiting HIV-1 infection in 80% of infected cells against the positive control.

thumbnailFigure 4. Time of intervention in HIV-1 life cycle. HeLa/CD4-LTR-β-gal cells were infected with HIV-1IIIB cell-free virus before A) bDLE (2 IU), B) T-20 (100 μM), C) UC781 (70 nM), D) 118-D-24 (120 μM) and E) Amprenavir (0.1 mM), were added upon HIV-1 inoculation (time zero) or at various time points post-infection. β-gal activity was measured following 24 hr of incubation. Percentage values are relative to the positive control (infected cells without drug pretreatment). The data represent the means ± standard deviations from three separate experiments, each of which was carried out in duplicate.

Cell protection assays

HeLa-CD4-LTR-β-gal cells were pretreated with bDLE for 30 minutes, which was then removed from the extracellular compartment with three washes, and subsequently the HeLa-CD4-LTR-β-gal cells were exposed to HIV-1IIIB for different pretreatment times (1, 5, 7, 10, 24 and 48 hours). As shown in Figure 5, HIV-1 infection was inhibited, as host cells were protected even after 7 hours from the bDLE removal.

thumbnailFigure 5. Cell protection against HIV-1 infection. β-gal activity was measured after HeLa/CD4-LTR-β-gal cells were exposed to bDLE (2 IU) for 30 minutes, washed and exposed to HIV-1IIIB cell-free virus at various time points post-treatment. Percentage values are relative to the positive control (infected cells without drug pretreatment). The data represent the means ± standard deviations from three separate experiments, each of which was carried out in duplicate.

Discussion

Due to the DLE immunomodulatory properties discussed before, progression to AIDS in asymptomatic HIV-1 infected individuals treated with conventional anti-retrovirals, has shown a retarded progression to AIDS under adjuvant treatment with dialyzable leukocyte extract (DLE), demonstrated by lower incidences of opportunistic infections and improved cellular immunity [9,38,44]. DLE simultaneously shows anti-HIV activity [44,47,48], modulates different types of immune effectors (e.g., cytokines and transcription factors)[11,47,49] and restores leukocyte subsets in treated patients [16,29,35,37]. All these properties make DLE a potential drug to be used in a therapeutic combination with antiretrovirals to improve immune and clinical responses.

Bovine dialyzable leukocyte extract (bDLE) is defined as the dialyzate of a heterogeneous mixture of low molecular weight substances released from disintegrated blood leukocytes or lymphoid tissue obtained from homogenized bovine spleen. Previous studies have shown inhibition of HIV-1 infection by suppression of the activity of essential transcription factors [42-44] and cytokines by DLE. The purpose of this study was to demonstrate the mechanism of antiviral action of bDLE in vitro in the inhibition of HIV-1 and the protection of host cells from infection.

First, we compared the half cytotoxic concentration of bDLE when exposed to HeLa-CD4-LTR-β-gal cells (CC50 = 3.41 IU) (Figure 1A) with the concentration of bDLE at which HIV-1IIIB infectivity was inhibited by 50% (IC50 = 1.33 IU) (Figure 1B) [48]. Then, the therapeutic index was determined (TI = 2.56) and used as an indicator of bDLE overall efficacy and safety. Despite the fact that TI was lower than expected (< 10), bDLE is a compound that has been used in clinical assays for more than fifty years without adverse reactions [10]. Better understanding of its inhibition mechanism can contribute to the development of new and improved anti-HIV-1 agents, which could be more efficient and have lower cytotoxicity. Furthermore, the residual infectivity [50,51] of cell-free viruses after bDLE treatment showed no inhibitory activity (Figure 2), which suggests that bDLE does not act as a virucide on the viral membrane to inhibit infection. bDLE showed inhibition of fusogenic cell-cell interactions in a dose-dependent manner when bDLE was exposed to CD4 cells, bDLE was then removed and the CD4 cells were mixed with Env cells. However, after 30 minutes pretreatment of Env cells with bDLE and then transfer to CD4 cells, there was no inhibition of Env-CD4 cell fusion. This observation further supported our previous results that bDLE acted on CD4 expressing cells and not on the Env of the HIV-1 virus. Furthermore, when exposed to CD4 cells for only 30 minutes, which is the time required for conformational changes in gp120 after CD4 binding [52], bDLE again showed inhibition in a dose-dependent manner (Figure 3A). These data suggested that bDLE acted on CD4 cells to inhibit HIV-1 infection. Antiviral results reported by Fernandez-Ortega et al. [53,54] have also contributed to the knowledge of the molecular mechanisms responsible for the effectiveness of bDLE against HIV infection.

To further determine the antiviral target of bDLE, a time-of-addition experiment was used to define the stage(s) of the viral life cycle that are blocked by these compounds. These results were compared with several antiretroviral drugs as controls that marked different stages of the viral cycle [55-61] (Figure 4B-E). Our findings suggested that bDLE highly inhibited HIV-1 infection at all stages (Figure 4A), possibly due to viral Tat protein down-regulation (Tat activates β-galactosidase indicator gene expression in HeLa-CD4-LTR-β-gal cells). Inhibition of HIV-1 Tat activity correlates with down-regulation of bcl-2 [62], but the action of bDLE on bcl-2 has not yet been determined in HIV-1 studies. Previously, bcl-2 was found to be reduced in breast cancer cell lines when treated with bDLE [32]. Furthermore, DLE inactivated the NF-κB signaling pathway by reducing the secretion of cytokines, such as IL-1 and TNF-α, which are effective inducers of NF-κB activity [63]. In HIV-infected T cells, NF-κB-dependent transactivation is essential for HIV-LTR induction. Interestingly, even the function of HIV Tat in resting CD4 T lymphocytes depends on κB responsive elements in the LTR [46]. Based on these interesting findings, it will be necessary to focus specifically on the transcriptional factors (NF-κB and SP1) and pro-apoptotic genes (bcl-2) in future research on bDLE as an antiviral against HIV-1 infection.

Lastly, bDLE was capable of rendering CD4 expressing cells resistant against HIV-1 infection by residual active virus for several hours [64]. Previous results indicated that, although pretreatment of cells (MT-4) with DLE for 3 hours had no effect, inhibition of HIV-1 production was observed when cells were pre-treated for a longer period of time (from 1 to 7 days), an effect that was characterized by the decline in TNFα and TGFβ1 gene expression and inhibition of transcriptional factors [9]. In our assays, after pretreatment of the HeLa-CD4-LTR-β-gal cells and bDLE removal prior to viral challenge, protection against infection lasted 7 hours after bDLE was removed from the extracellular compartment (Figure 5). These results indicated that bDLE could induce long-term viral inhibition through cell protection, as well as modulate cell susceptibility to viral infection in vitro, in agreement with previously reported data on DLE obtained from human donors by molecular methods [9].

Conclusion

The data presented here were novel in that they proved that bDLE acted by inhibiting HIV-1 infection through protection of the host target CD4 cells at noncytotoxic levels. This effect was found to be modulated through transcriptional factors (NF-κB and SP1) necessary for HIV-1 replication. In addition, bDLE was discovered to act through all viral cycle stages to protect cells from HIV-1 infection for hours without affecting the HIV membrane. Based on our results obtained above, bDLE should be further studied to determine its potential use as a therapeutic agent in HIV-1 infection, especially due to its long-lasting cell protection against HIV-1 infection and lack of negative side effects.

Methods

Reagents, cells and HIV-1 isolates

The following reagents were obtained through the AIDS Research and Reference Reagent Program (NIH): HeLa-CD4-LTR-β-gal cells from Dr. Michael Emerman; HL2/3 cells from Dr. Barbara K. Felber and Dr. George N. Pavlakis; HIV-1IIIB, fusion inhibitor T-20, integrase inhibitor 118-D-24 and protease inhibitor Amprenavir from Dr. Suzanne Gartner, Dr. Mikulas Popovic and Dr. Robert Gallo. UC781, a no nucleoside reverse-transcriptase inhibitor (NNRTI), was kindly donated by Dr. Gadi Borkow. The bDLE used in our study was produced by the Laboratory of Immunology and Virology at the Universidad Autonoma de Nuevo Leon, Mexico, following a modified process described by Lawrence et al. [9]. The bDLE was lyophilized, tested for endogenous pyrogens using the Limulus amebocyte lysate assay (MP Biomedicals, Inc.), and determined to be free of bacterial contamination by culturing in media and in vivo mice inoculations. The bDLE obtained from 15 × 108 leukocytes was defined as one unit (1 Unit)[37].

Cytotoxicity Assays

A stock solution of bDLE was diluted two-fold diluted in growth medium and subsequently added into wells containing 5 × 104 HeLa-CD4-LTR-β-gal cells. Microtiter plates were incubated at 37°C in a 5% CO2 air humidified atmosphere for 24 hours. Assessments of cell viability were carried out using a CellTiter-Glo® Luminescent Cell Viability Assay (Promega). Cytotoxicity was evaluated based on the percentage cell survival relative to the result obtained in the absence of any compound.

HIV-1 Infection Inhibition Assays

Serial two-fold dilutions of bDLE were mixed with 105 TCID50 of HIV-1IIIB and added to the wells containing 5 × 104 HeLa-CD4-LTR-β-gal cells with a multiplicity of infection (MOI) of 0.2 - 0.5. HIV-1 infection was assessed after 24 hours of incubation by quantifying the activity of the β-galactosidase produced after infection with the Beta-Glo Assay System (Promega). The 50% inhibitory concentration (IC50) was defined according to the percentage of infection inhibited relative to the positive control.

Virucidal Activity Assay

Serial two-fold dilutions of bDLE were added to HIV-1IIIB (T tropic virus) and HIV-1Ba-L (M Tropic) cell-free virus. After incubation for 5 min at room temperature, the mixtures were centrifuged three times at 10, 000 rpm, the supernatant fluids removed, and the pellets washed three times. The final pellets were resuspended in Dulbecco's Modified Eagle Medium (DMEM) and placed into 96-well plates with HeLa-CD4-LTR-β-gal cells. The cells were incubated in a 5% CO2 humidified incubator at 37°C for 24 h. Assessment of HIV-1 infection was made with the Beta-Glo Assay System. The percentage of residual infectivity after bDLE treatment was then calculated with respect to the positive control of untreated virus.

Cell-based Fusion Assay

HeLa-derived HL2/3 cells (Env cells), which express the HIV-1HXB2 Env, Tat, Gag, Rev, and Nef proteins, were co-cultured with HeLa-CD4-LTR-β-gal cells (CD4 cells) at a 1:1 cell density ratio (5 × 104 cells/well each) for 24 h in the absence or presence of two-fold dilutions of bDLE, UC781, and T-20 in order to examine whether the compounds interfered with the binding process of HIV-1 Env and the CD4 receptor. Also, both HeLa-CD4-LTR-β-gal and HL2/3 cells were exposed to the aforementioned compounds for only 30 minutes and then washed twice to eliminate residual compound before co-cultivating with the other cell line. Upon fusion of both cell lines, the Tat protein from HL2/3 cells activated β-galactosidase (β-gal) indicator gene expression in HeLa-CD4-LTR-β-gal cells [41]. β-gal activity was quantified with the Beta-Glo Assay System (Promega). The percentage of inhibition of HL2/3-HeLa CD4 cell fusion was calculated with respect to the positive control of untreated cells.

Time of Addition Experiments

HeLa-CD4-LTR-β-gal cells were infected with 105 TCID50 of HIV-1IIIB cell-free virus with a 0.2-0.5 MOI. bDLE (2 IU), T-20 (100 μM), UC781 (70 nM), 118-D-24 (120 μM) and Amprenavir (0.1 mM) were then added upon HIV-1 inoculation (time zero) or at various time points post-inoculation. The reference compounds were added at a concentration several times their EC50 for infectivity of HIV-1IIIB. Infection inhibition was quantified after 24 h by measuring β-gal activity with the Beta-Glo Assay System.

Cell Protection Assays

HeLa-CD4-LTR-β-gal cells were incubated with bDLE (2 Units) for 30 minutes and subsequently washed with PBS three times. Then, the cells were exposed to 105 TCID50 of HIV-1IIIB cell-free virus with a 0.2-0.5 MOI for different times (1, 5, 7, 10, 24 and 48 h). Infection inhibition was quantified after 24 h by measuring β-gal activity with the Beta-Glo Assay System.

Statistical analysis

Graphs were done with SigmaPlot 10.0 software and the values shown are means ± standard deviations from three separate experiments, each of which was carried out in duplicate. Cytotoxicity and inhibition assessment graphs are linear regression curves done with SigmaPlot 10.0 software.

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

All authors read and approved the final manuscript. HHL participated in the conception and experimental design of the in vitro HIV-1 manipulation and infection assays, in the analysis and interpretation of the data, and in the writing and revision of this report. LI-T participated in the analysis and interpretation the results. HHL and LI-T made equal contributions to this study. EN-GT participated in the analysis and interpretation of the data and in writing and revising this report. SM-FT participated in the analysis and writing of the report. JIB participated in revising this report. CR-P participated in the experimental design of this research.

Acknowledgements

The following funding sources supported our experiments: the Programa de Apoyo a la Investigacion en Ciencia y Tecnologia (PAICyT) of the Universidad Autonoma de Nuevo Leon, Mexico, and the Consejo Nacional de Ciencia y Tecnologia (CONACyT) of Mexico.

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