Table 1

Summary of evaluation criteria for the RNA-isolation protocol

Experiment

#

Biopsy weight*

RNA yield

RIN value

aRNA yield**$

Array signal**


threshold

-

0.1 μg Affymetrix

0.2 μg NimbleGen

6.5 for both platforms

10.0 μg Affymetrix

2.0 μg NimbleGen

-


avg.

min.

avg.

min.

avg.

min.

avg.

min.

avg.

min.


Human

97

8.2 mg

3.2 mg

1.5 μg

0.2 μg

8.1

3.3

35.6 μg

10.0 μg

7.8

5.6


Mouse

16

ND

ND

2.4 μg

0.6 μg

7.5

6.5

4.2 μg

2.9 μg

11.0

10.5


ND: not determined

* 44 half human biopsies were weighted.

** 12 mouse biopsies were analyzed by microarray technology

$ Platform dependent results and thresholds.

¥ Array averaged log2 probe intensity

Bruning et al. BMC Research Notes 2011 4:438   doi:10.1186/1756-0500-4-438

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