Open Access Highly Accessed Research article

Selection of reference genes for qPCR in hairy root cultures of peanut

Jose Condori13, Cesar Nopo-Olazabal1, Giuliana Medrano1 and Fabricio Medina-Bolivar12*

Author Affiliations

1 Arkansas Biosciences Institute, Arkansas State University, P.O. Box 639, State University, AR 72467, USA

2 Department of Biological Sciences, Arkansas State University, P.O. Box 599, State University, AR 72467, USA

3 BioStrategies LC, P.O. Box 2428, State University, AR 72467, USA

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BMC Research Notes 2011, 4:392  doi:10.1186/1756-0500-4-392

Published: 10 October 2011

Additional files

Additional file 1:

Summary of reference genes in literature used in plants and humans1. Select literature used in identifying potential validation of reference genes candidates are listed by first author and year of the publication. Gene targets used for each study are indicated by accession number (GenBank or TAIR database). One reference1 provides reference genes used for humans. The gene(s) identified as best reference gene(s) in each of the cited studies is showed in red. In green: Genes selected from Transcript arrays in Arabidopsis [13]. In yellow: Most frequently reference genes used.

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Additional file 2:

RNA chemical contaminants. Indicator of chemical contaminants in RNA was measured using the ratio of: absorbance at 260 nm/absorbance at 230 nm. X-axis shows the intervals of A260/A230 for the 60 biological samples from the time course experiments.

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Additional file 3:

Primer specificity of reference genes for APT1, UBQ11 and AP47. Melting curves generated for 40 (red), 8 (green), 1.6 (light blue), 0.32 (yellow) and 0.064 (black) ng of cDNA.

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Additional file 4:

Primer specificity of reference genes for ACT7, TIP41 and H3. Melting curves generated for 40 (red), 8 (green), 1.6 (light blue), 0.32 (yellow) and 0.064 (black) ng of cDNA.

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Additional file 5:

Primer specificity of reference genes for TBP2, EFa1 and RPL8C. Melting curves generated for 40 (red), 8 (green), 1.6 (light blue), 0.32 (yellow) and 0.064 (black) ng of cDNA.

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Open Data

Additional file 6:

Primer specificity of reference genes for EIF4A1, CYP1 and rolC. Melting curves generated for 40 (red), 8 (green), 1.6 (light blue), 0.32 (yellow) and 0.064 (black) ng of cDNA.

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Open Data

Additional file 7:

Primer specificity of reference genes for AT1G31300, HEL and GAPDH. Melting curves generated for 40 (red), 8 (green), 1.6 (light blue), 0.32 (yellow) and 0.064 (black) ng of cDNA.

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Open Data

Additional file 8:

Primer specificity of reference genes for TUA3, SAND and RPB1. Melting curves generated for 40 (red), 8 (green), 1.6 (light blue), 0.32 (yellow) and 0.064 (black) ng of cDNA.

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Open Data

Additional file 9:

Primer specificity of reference genes for AT4G33380, PP2AA3 and aux1. Melting curves generated for 40 (red), 8 (green), 1.6 (light blue), 0.32 (yellow) and 0.064 (black) ng of cDNA.

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Open Data