Table 3

Details of the mutations found by TILLING in the gene targets screened in the two EMS-mutagenized melon populations, Piel de Sapo (PS) and Charentais (CH)

Gene/population

Amplicon size (bp)

Identified mutants

1

Exon/Intron

Position

AA change

M2 Family

M2 segregation (WT/H/M)2

SIFT score


Cm-eIF4E/PS

500

1

1

Exon 1

A 74 G

R 25 K

F2036

9/7/8

0.00


1169

3

2

Exon 2

G 385 A

E 129 K

F57

5/3/0

0.00


3

Exon 2

G 465 A

T 155 T

F1880


4

Intron 2

G/C

F959


Cm-eIF4E/CH

500

3

5

Exon 1

G 289 A

E 97 K

53

0.02


6

Exon 1

C 294 T

F 98 F

2532


7

Intron 1

G/A

1420


1169

11

8

Exon 2

C 401 T

A 134 V

1229

0.10


9

Exon 2

G 407 A

G 136 E

444

0.00


10

Intron 2

C/A

1287


11

Intron 2

C/T

1115


12

Intron 3

G/A first nt of intron

splicing

1667


13

Intron 3

C/T

2646


14

Intron 3

C/T

1233


15

Exon 4

G 616 A

E 206 K

2060

0.09


16

Intron 4

C/T

2035


17

3'UTR

T/C

1368


18

3'UTR

G/A

2163


222 TGA


CmeIF(iso)4E/PS

1014

0

-


CmeIF(iso)4E/CH

1014

1

19

Exon 1

G 128 A

R 43 K

M1587

1.00


Cm-PDS/PS

1188

2

20

Intron 1

C/T

F1151

4/7/4


21

Exon 2

C 560 T

S 227 F

C384

6/9/0

0.00


Cm-PDS/CH

1188

4

22

Exon 1

C 395 T

S 172 F

1182

0.01


23

Intron 1

C/T

2495


24

Intron 1

T/C

1460


25

Intron 1

C/T

1226


Cm-ACO1/PS

933

0

-


933

1

26

Exon 4

C 728 T

T 243 I

C142

10/6/3

1.00


Cm-NOR/PS

1272

0


Cm-DET1/PS

1185

1

27

Exon 2

T 402 A

L 135 L

I76


776

2

28

Exon 4

G 760 A

V 255 I

F201

2/11/2

0.37


29

Exon 4

C 848 T

S 284 L

F1876

2/4/3

0.25


1232

2

30

Intron 6

G 2981 A

F1248


31

Intron 7

C 3211 T

C487


Cm-DHS/PS

578

1

32

Exon 1

G 184 A

V 62 I

F 1728

13/20/0

0.58


754

1

33

Exon 3

G 610 A

V 204 I

C348

2/6/0

0.27


1Number of mutations corresponds to those represented in Figures 1 and 2

2 For some mutations, it was difficult to obtain homozygous MM plants as seedlings showed altered development.

González et al. BMC Research Notes 2011 4:289   doi:10.1186/1756-0500-4-289

Open Data