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Open Access Correspondence

Transgenic tobacco plants constitutively expressing Arabidopsis NPR1 show enhanced resistance to root-knot nematode, Meloidogyne incognita

D Bhanu Priya1, N Somasekhar2, JS Prasad2 and PB Kirti1*

Author Affiliations

1 Department of Plant Sciences, University of Hyderabad, Hyderabad 500046, India

2 Directorate of Rice Research, Rajendranagar, Hyderabad 500046, India

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BMC Research Notes 2011, 4:231  doi:10.1186/1756-0500-4-231

Published: 4 July 2011

Abstract

In Arabidopsis, non-expressor of pathogenesis related genes-1, NPR1 has been shown to be a positive regulator of the salicylic acid controlled systemic acquired resistance pathway and modulates the cross talk between SA and JA signaling. Transgenic plants expressing AtNPR1 constitutively exhibited resistance against pathogens as well as herbivory. In the present study, tobacco transgenic plants expressing AtNPR1 were studied further for their response to infection by the sedentary endoparasitic root knot nematode, Meloidogyne incognita. Transgenic plants showed enhanced resistance against the root-knot nematode infection. Prominent differences in the shoot and root weights of wild type and transgenic plants were observed post-inoculation with M. incognita. This was associated with a decrease in the number of root galls and egg masses in transgenic plants compared to WT. The transgenic plants also showed constitutive and induced expression of some PR protein genes, when challenged with M. incognita.

Keywords:
Transgenic tobacco; AtNPR1; constitutive expression of PR proteins; nematode resistance; Meloidogyne incognita