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Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc

Dongrim Seol12, Hyeonghun Choe12, Hongjun Zheng1, Keewoong Jang12, Prem S Ramakrishnan1*, Tae-Hong Lim2 and James A Martin1

Author Affiliations

1 Department of Orthopedics and Rehabilitation, University of Iowa, 1182 ML, Iowa city, IA 52242, USA

2 Department of Biomedical Engineering, University of Iowa, 1402 SC, Iowa city, IA 52242, USA

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BMC Research Notes 2011, 4:162  doi:10.1186/1756-0500-4-162

Published: 26 May 2011

Additional files

Additional file 1:

Melting curve analysis. Melting curve analysis of 7 candidate reference genes using ABI PRISM 7700 Sequence Detection System (Applied Biosystems) with a Sequence Detection System (SDS) software version 2.3.

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Open Data

Additional file 2:

MMP-3 gene expression in the rat intervertebral disc. Relative expression level of MMP-3 in the rat intervertebral disc normalized by common used reference genes (Actb, GAPDH and 18S rRNA) and an optimal combination of reference genes (Hprt1 and CycA).

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Open Data