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Applying neutral drift to the directed molecular evolution of a β-glucuronidase into a β-galactosidase: Two different evolutionary pathways lead to the same variant

Wendy S Smith, Jennifer R Hale and Cameron Neylon*

BMC Research Notes 2011, 4:138  doi:10.1186/1756-0500-4-138

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Minor correction to sequence data

Cameron Neylon   (2014-03-25 11:12)  PLOS

In table 1 the generation of two variant β-glucuronidases with increased β-galactosidase activity (labelled as C4.1 and C4.2) is described. Further analysis of the sequencing of these mutants has revealed a minor error in the listed mutation sites. Mutant C4.1 also contains a silent mutation. Mutant C4.2 is missing mutation sites N166T and N412D and the listing of a silent mutation. These make the mutants:

C4.1: G245A/K370R/W529L

C4.2: V67D/N166T/K370R/N412D/W529L

These changes do not affect the overall results and conclusions of our report.

Cameron Neylon on behalf of the authors.

Competing interests

No competing interests.


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