Additional file 1.

Supplemental Figure S1: Scheme of mouse olfactory epithelium and dorsal immunolabeling. A. OMACS immunolabeling of the dorsal region (green). Blue indicates nuclear staining. The images were obtained by two-photon microscopy (Zeiss/BioRad Radiance 2100MP coupled with a Coherent Chameleon Ultra) of the intact olfactory epithelium at 955 nm excitation and using standard blue and green emission filter sets. Images are maximum Z-projections of 10-20 images at 5 micron steps. Each image is a Kalman average (n = 4) acquired at 16-bit resolution. Post-processing was accomplished with NIH ImageJ. B. The scheme of the sagittal view of the left hemisphere of mouse olfactory epithelium. A dorsal region, zone I is colored in yellow and ventral region (endoturbinates (IIa, IIb, III and IV) in orange. OB-olfactory bulb. Dashed lines indicate sites of immunostaining images of anterior (A), middle (M) and posterior (P) part of dorsal region (A).

Format: TIFF Size: 694KB Download file

Abaffy and DeFazio BMC Research Notes 2011 4:137   doi:10.1186/1756-0500-4-137