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Open Access Short Report

Distribution study of Chlamydia trachomatis genotypes in symptomatic patients in Buenos Aires, Argentina: association between genotype E and neonatal conjunctivitis

Lucía Gallo Vaulet12*, Carolina Entrocassi23, Ana I Corominas2 and Marcelo Rodríguez Fermepin12

Author Affiliations

1 Inmunología Clínica, Departamento de Bioquímica Clínica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina

2 Unidad de Estudios de Chlamydia y otras Infecciones del Tracto Genital, INFIBIOC - Instituto de Fisiopatología y Bioquímica Clínica, Universidad de Buenos Aires, Argentina

3 Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina

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BMC Research Notes 2010, 3:34  doi:10.1186/1756-0500-3-34

Published: 9 February 2010

Abstract

Background

Chlamydia trachomatis infections are the most prevalent sexually transmitted bacterial infections in the world. There is scarce data available referring to the distribution of C. trachomatis genotypes in Argentina. The aim of this study was to identify the genotypes of C. trachomatis circulating in the metropolitan area of Buenos Aires (Argentina) associated with ophthalmia neonatorum and genital infections.

Findings

From 2001 to 2006, 199 positive samples for C. trachomatis infection from symptomatic adult patients and neonates with ophthalmia neonatorum from two public hospitals were studied. C. trachomatis genotypes were determined by PCR-RFLP of an ompA fragment.

Genotype E was the most prevalent regardless of the sample origin (46.3% 57/123 in adults and 72.4% 55/76 in neonates), followed by genotype D (19.5% 24/123) and F (14.6% 18/123) in adults, and G (9.2% 7/76) and D (7.9% 6/76) in neonates. We detected a significantly higher frequency of genotype E (p < 0.001, OR = 3.03 (1.57<OR<5.90)) in ophthalmia neonatorum than in genital specimens. Genotype D was associated with genital localization (p < 0.05, OR = 2.83 (1.03<OR<8.18)).

Conclusion

We found a particularly increased frequency of C. trachomatis genotype E in neonatal conjunctivitis, which may indicate an epidemiological association between this genotype and the newborn population. The present study also contributed to increase the knowledge on genotype distribution of Chlamydia trachomatis in symptomatic adult patients in Buenos Aires, Argentina, in which genotypes E, D and F were the predominant ones.