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Open Access Data Note

High-throughput molecular identification of Staphylococcus spp. isolated from a clean room facility in an environmental monitoring program

Norhan S Sheraba1, Aymen S Yassin2* and Magdy A Amin2

Author Affiliations

1 VACSERA, The Holding Company for Biological Products & Vaccines, Giza, 22311, Egypt

2 Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt

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BMC Research Notes 2010, 3:278  doi:10.1186/1756-0500-3-278

Published: 4 November 2010

Abstract

Background

The staphylococci are one of the most common environmental isolates found in clean room facility. Consequently, isolation followed by comprehensive and accurate identification is an essential step in any environmental monitoring program.

Findings

We have used the API Staph identification kit (bioMérieux, France) which depends on the expression of metabolic activities and or morphological features to identify the Staphylococcus isolates. The API staphylococci showed low sensitivity in the identification of some species, so we performed molecular methods based on PCR based fingerprinting of glyceraldehyde-3-phosphate dehydrogenase encoding gene as useful taxonomic tool for examining Staphylococcus isolates.

Conclusions

Our results showed that PCR protocol used in this study which depends on genotypic features was relatively accurate, rapid, sensitive and superior in the identification of at least 7 species of Staphylococcus than API Staph which depends on phenotypic features.