Table 1

Enzymatic characterization

CNPase construct

Lee et al.[19]

1-1 (500 ng)

2-1 (5 ug)

3-1 (500 ng)

3-2 (500 ng)

Full-length

Catalytic fragment


Km (μM)

193 ± 26

537 ± 14

445 ± 41

406 ± 11

263 ± 12

295 ± 22

Kcat (s-1)

270 ± 10

118 ± 1.1

570 ± 19

612 ± 6.1

836 ± 11

1690 ± 39

Kcat/Km

1.40

0.22

1.28

1.51

3.2

5.7


Myllykoski and Kursula BMC Research Notes 2010 3:12   doi:10.1186/1756-0500-3-12

Open Data