Research article
TILLING in the two-rowed barley cultivar 'Barke' reveals preferred sites of functional diversity in the gene HvHox1
1 Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany
2 Saarland University, Dept. Of Biosciences-Botany, Campus A2.4, D-66123 Saarbrücken, Germany
3 National Institute of Agrobiological Sciences (NIAS), Plant Genome Research Unit, Kannondai 1-2-1, Tsukuba, Ibaraki 305 8602, Japan
4 Nordic Genetic Resource Centre, P.O. Box 41, SE-230 53 Alnarp, Sweden
BMC Research Notes 2009, 2:258 doi:10.1186/1756-0500-2-258
Published: 17 December 2009Additional files
Additional file 1:
Additional figure 1 - Examples of chlorophyll-deficient seedling mutants in M2. Supplemental figure showing chlorophyll-deficient seedling mutants. Mutants belonging to the unicolour sub-class: (A) albina, (B) xantha, and (C) viridis. Mutants belonging to the bicolour sub-class: (D) viridoalbina and (E) striata. The classification corresponds to [20].
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Additional file 2:
Additional figure 2 - Frequency of mutant phenotype categories observed in M2 and M3 screenings. This supplemental figure is an overview of the occurrence of different mutant phenotypes observed in the mutant populations. Mutant phenotypes observed in M2 and M3 derived from mutagenesis at 20 - 35 mM EMS were classified into six morphological categories. (A) In the M2 generation, approximately 20% of the 12,703 individuals showed a mutant phenotype vs. the 'Barke' wild-type. (B) In the M3 generation, 1,200 M3 families (16 plants per M3 line) were grown, and approximately 37% of the total M3 families displayed a mutant phenotype vs. the 'Barke' wild-type.
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Additional file 3:
Additional table 1 - A series of mutations in the HvHox1 gene identified by TILLING. The additional table shows the position and mutation types identified in the HvHox1 gene.
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Additional file 4:
Additional table 2 - Sequences of primers used for TILLING of candidate genes. This additional table specifies primers used for PCR amplification of DNA pools and sequencing.
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