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Synergistic effect of methyljasmonate and cyclodextrin on stilbene biosynthesis pathway gene expression and resveratrol production in Monastrell grapevine cell cultures

Diego Lijavetzky12, Lorena Almagro3, Sarai Belchi-Navarro3, José M Martínez-Zapater14, Roque Bru5 and Maria A Pedreño3*

  • * Corresponding author: Maria A Pedreño mpedreno@um.es

  • † Equal contributors

Author Affiliations

1 Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), C/Darwin 3, 28049 Madrid, Spain

2 Cátedra de Química Orgánica y Biológica, Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), M5528AHB Chacras de Coria, Argentina

3 Departamento de Biología Vegetal, Facultad de Biología, Universidad de Murcia, Campus de Espinardo, E-30100 Murcia, Spain

4 Instituto de Ciencias de la Vid y el Vino (Consejo Superior de Investigaciones Científicas, Universidad de La Rioja, Gobierno de La Rioja), Campus de la Universidad de La Rioja, C/Madre de Dios 51, 26006 Logroño, Spain

5 Departamento de Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, Apartado 99, E-03080 Alicante, Spain

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BMC Research Notes 2008, 1:132  doi:10.1186/1756-0500-1-132

Published: 22 December 2008

Abstract

Background

Plant cell cultures have been shown as feasible systems for the production of secondary metabolites, being the elicitation with biotic or abiotic stimuli the most efficient strategy to increase the production of those metabolites. Vitaceae phytoalexins constitute a group of molecules belonging to the stilbene family which are derivatives of the trans-resveratrol structure and are produced by plants and cell cultures as a response to biotic and abiotic stresses. The potential benefits of resveratrol on human health have made it one of the most thoroughly studied phytochemical molecules. The aim of this study was to evaluate the elicitor effect of both cyclodextrin (CD) and methyljasmonate (MeJA) on grapevine cell cultures by carrying out a quantitative analysis of their role on resveratrol production and on the expression of stilbene biosynthetic genes in Vitis vinifera cv Monastrell albino cell suspension cultures.

Findings

MeJA and CD significantly but transiently induced the expression of stilbene biosynthetic genes when independently used to treat grapevine cells. This expression correlated with resveratrol production in CD-treated cells but not in MeJA-treated cells, which growth was drastically affected. In the combined treatment of CD and MeJA cell growth was similarly affected, however resveratrol production was almost one order of magnitude higher, in correlation with maximum expression values for stilbene biosynthetic genes.

Conclusion

The effect of MeJA on cell division combined with a true and strong elicitor like CD could be responsible for the observed synergistic effect of both compounds on resveratrol production and on the expression of genes in the stilbene pathway.