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Open Access Research article

A systems biology approach to understand the pathophysiological mechanisms of cardiac pathological hypertrophy associated with rosiglitazone

Lars Verschuren1*, Peter Y Wielinga2, Thomas Kelder1, Marijana Radonjic1, Kanita Salic2, Robert Kleemann2, Ben van Ommen1 and Teake Kooistra2

Author Affiliations

1 TNO, Department Microbiology and Systems Biology, P.O. Box 360, 3704 AJ Zeist, The Netherlands

2 TNO, Department Metabolic Health Research, Leiden, The Netherlands

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BMC Medical Genomics 2014, 7:35  doi:10.1186/1755-8794-7-35

Published: 17 June 2014

Abstract

Background

Cardiac pathological hypertrophy is associated with a significantly increased risk of coronary heart disease and has been observed in diabetic patients treated with rosiglitazone whereas most published studies do not suggest a similar increase in risk of cardiovascular events in pioglitazone-treated diabetic subjects. This study sought to understand the pathophysiological and molecular mechanisms underlying the disparate cardiovascular effects of rosiglitazone and pioglitazone and yield knowledge as to the causative nature of rosiglitazone-associated cardiac hypertrophy.

Methods

We used a high-fat diet-induced pre-diabetic mouse model to allow bioinformatics analysis of the transcriptome of the heart of mice treated with rosiglitazone or pioglitazone.

Results

Our data show that rosiglitazone and pioglitazone both markedly improved systemic markers for glucose homeostasis, fasting plasma glucose and insulin, and the urinary excretion of albumin. Only rosiglitazone, but not pioglitazone, tended to increase atherosclerosis and induced pathological cardiac hypertrophy, based on a significant increase in heart weight and increased expression of the validated markers, ANP and BNP. Functional enrichment analysis of the rosiglitazone-specific cardiac gene expression suggests that a shift in cardiac energy metabolism, in particular decreased fatty acid oxidation toward increased glucose utilization as indicated by down regulation of relevant PPAR╬▒ and PGC1╬▒ target genes. This underlies the rosiglitazone-associated pathological hypertrophic cardiac phenotype in the current study.

Conclusion

Application of a systems biology approach uncovered a shift in energy metabolism by rosiglitazone that may impact cardiac pathological hypertrophy.