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Open Access Research article

DNA methylation study of fetus genome through a genome-wide analysis

Hong-Dan Wang12, Qiao-Fang Hou1, Qian-Nan Guo1, Tao Li1, Dong Wu1, Xian-Ping Zhang3, Yan Chu1, Miao He1, Hai Xiao1, Liang-Jie Guo1, Ke Yang1, Shi-Xiu Liao1* and Bo-Feng Zhu2*

Author Affiliations

1 Medical Genetic Institute of Henan Province, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou 450003, PR China

2 School of Medicine, Xi’an Jiaotong University, Xi’an 710061, PR China

3 Armed police Guangxi Corps Hospital, Nanning 530003, PR China

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BMC Medical Genomics 2014, 7:18  doi:10.1186/1755-8794-7-18

Published: 15 April 2014

Abstract

Background

DNA methylation is a crucial epigenetic modification of the genome which is involved in embryonic development, transcription, chromatin structure, X chromosome inactivation, genomic imprinting and chromosome stability. Consistent with these important roles, DNA methylation has been demonstrated to be required for vertebrate early embryogenesis and essential for regulating temporal and spatial expression of genes controlling cell fate and differentiation. Further studies have shown that abnormal DNA methylation is associated with human diseases including the embryonic development diseases. We attempt to study the DNA methylation status of CpG islands in fetus related to fetus growth and development.

Methods

GeneChip® Human Tiling 2.0R Array set is used for analysis of methylated DNA in a whole-genome wide in 8 pairs amniotic fluid and maternal blood DNA samples.

Results

We found 1 fetus hypermethylation DNA markers and 4 fetus hypomethylation DNA markers though a Genome-wide analysis. These DNA markers all found to be associated with the critical genes for fetus growth and development (SH2D3C gene, EML3 gene, TRIM71 gene, HOXA3 gene and HOXA5 gene).

Conclusions

These genes can be used as a biomarker for association studying of embryonic development, pathological pregnancy and so on. The present study has provided new and fundamental insights into the roles that DNA methylation has in embryonic development and in the pathological pregnancy.

Keywords:
DNA methylation; Fetus; GeneChip® human tiling 2.0R array set; Clone sequencing; Embryonic development