Table 2

Comparison of results from microarrays and RT-qPCR
Large-MI Moderate-MI Small-MI
Results from RT-qPCR Results from microarrays Results from RT-qPCR Results from RT-qPCR

Gene

symbol

Fold change P-value Significance Fold change P-value Significance Fold change P-value Significance Fold change P-value Significance
Biomarkers in heart failure
End1 1.9 0.043 ** 1.3 0.005 ** -1.1 0.412 ns -1.3 0.147 ns
Nppa 19.2 0.001 *** 3.7 1.3e-5 *** 1.1 0.884 ns 1.2 0.694 ns
Nppb 2.2 0.001 *** 1.4 0.14 ns -1.1 0.849 ns -1.5 0.496 ns
Others related to cardiac hypertrophy and left ventricular remodelling
Corin 2.6 0.020 * 1.4 3e-5 *** 1 0.788 ns 1 0.934 ns
Hcn4 3.6 0.013 * 2.1 0.001 *** 1 0.951 ns -1.4 0.343 ns
Pparg -1.7 0.017 ** -1.4 1.4e-4 *** 1.1 0.643 ns 1 0.865 ns
Tgfβ1 1.4 0.071 ns 1.3 1.6e-5 *** 1.1 0.613 ns 1.1 0.712 ns
Tgfβ2 3.1 0.010 ** 2 7.8e-5 *** -1.1 0.762 ns 1 0.874 ns
ECM – Extracellular matrix components
ECM structural components
Col1a1 3.9 0.003 ** 2.3 6.1e-6 *** 1 0.767 ns 1 0.852 ns
Col1a2 2.3 0.011 ** 1.6 5e-5 *** -1.1 0.685 ns 1 0.852 ns
Col3a1 2.1 0.017 ** 1.5 1.3e-5 *** -1.1 0.579 ns 1.1 0.619 ns
Col8a1 5.1 0.001 *** 2.1 3.1e-8 *** 1 0.880 ns 1 0.390 ns
Col12a1 5.1 0.001 *** 2.9 3.4e-8 *** 1 0.912 ns -1.1 0.215 ns
Col14a1 2.4 0.001 *** 2 1.0e-7 *** 1.1 0.628 ns 1.1 0.891 ns
Col18a1 2.2 0.001 *** 1.3 7.6e-5 *** -1.1 0.668 ns -1.1 0.310 ns
Eln 2.2 0.001 *** 1.4 1.8e-5 *** 1.2 0.304 ns 1.1 0.689 ns
Fn1 3.8 0.005 ** 2.9 6.2e-8 *** 1.2 0.384 ns 1.1 0522 ns
Lamc1 1.3 0.014 ** 1.3 5.1e-7 *** 1.1 0.451 ns 1 0.773 ns
Prg4 2.1 0.004 ** 1.7 2.4e-5 *** 1 0.907 ns -1.1 0.430 ns
Vtn -1.4 0.015 * -1.4 2.3e-6 *** 1 0.874 ns -1.1 0.167 ns
Matricellular proteins
Mmp2 1.6 0.001 *** 1.4 7.5e-7 *** 1 0.855 ns 1.1 0.343 ns
Mmp9 2.7 0.351 ns 1.73 0.037 * 1.2 0.660 ns 1 0.871 ns
Mmp13 62.2 0.001 *** 2 0.021 * -1.2 0.358 ns 1 0.862 ns
Mmp14 2.3 0.019 ** 1.3 0.002 ** 1.1 0.529 ns -1.1 0.465 ns
Mmp16 2.9 0.020 ** 1.3 0.006 *** -1.4 0.204 ns -1.3 0.334 ns
Mmp23 1.8 0.027 ** 1.3 1.0e-4 *** -1.1 0.572 ns -1.2 0.438 ns
Postn 20.8 0.001 *** 8 1.2e-9 *** 1 0.988 ns 1.1 0.738 ns
Spp1 52.3 0.003 ** 4.1 2.3e-5 *** 2.7 0.157 ns 2.3 0.212 ns
Thbs4 19.4 0.003 ** 6.1 8e-9 *** -1.7 0.272 ns -1.3 0.348 ns
Timp1 3 0.008 ** 2.4 2.8e-7 *** 1.2 0.287 ns 1.4 0.041 *
Timp2 1.7 0.037 ** 1.5 4.6e-7 *** -1.1 0.666 ns 1 0.936 ns
Timp3 1.9 0.043 * 1.2 7.8e-5 *** -1.4 0.288 ns -1.3 0.321 ns
Timp4 -1.5 0.151 ns -1.4 0.006 ** -1.3 0.307 ns -1.1 0.716 ns

Expression level of selected genes was measured 2 months after operation in left ventricle tissues of all infarcted rats and compared to sham-operated group (statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001, ns = non significant, † expression level in sham-operated rats close to the limit of detection). Results were normalized to Gapdh and Hprt.

Tulacz et al.

Tulacz et al. BMC Medical Genomics 2013 6:49   doi:10.1186/1755-8794-6-49

Open Data