Open Access Highly Accessed Research article

Assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges

Suzan Wopereis2, Danielle Wolvers1, Marjan van Erk2, Michiel Gribnau1, Bas Kremer2, Ferdi A van Dorsten13, Esther Boelsma2, Ursula Garczarek1, Nicole Cnubben2, Leon Frenken1, Paul van der Logt1, Henk FJ Hendriks2, Ruud Albers145, John van Duynhoven135, Ben van Ommen2 and Doris M Jacobs13*

Author Affiliations

1 Unilever R&D, Olivier van Noortlaan 120, Vlaardingen 3130 AC, The Netherlands

2 TNO, Utrechtseweg 48, Zeist 3704 HE, The Netherlands

3 Netherlands Metabolomics Centre, Einsteinweg 55, 2333 CL, Leiden, The Netherlands

4 NutriLeads Innovations, Pancrasgorsedijk 7, Rockanje 3235 KT, The Netherlands

5 Wageningen University, Dreijenlaan 3, Wageningen 6703 HA, The Netherlands

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BMC Medical Genomics 2013, 6:44  doi:10.1186/1755-8794-6-44

Published: 27 October 2013

Additional files

Additional file 1: Table S1:

Demographic characteristics of subjects at inclusion. Table S2. Parent and product m/z values of LC-MS/MS analysis of eicosanoids. The eicosanoids in bold were selected for further processing. Table S3. Overview of selected genes related to inflammation and their primers; shaded genes are reference genes; genes in italic and with grey font did not pass quality control or were not considered for data analysis. Table S4. Gene-sets related to specialized biological functions and pathways selected from gene-array of a total of 85 genes. Table S5. Fold changes of genes. Figure S1. Statistical significance of repeated measurements, AUCmin, AUCplusand AUCtotal of inflammatory markers and clinical paramters (upper panel), oxylipins (middle panel) and gene expression data (lower panel) after OGTT, OLTT and OG + LTT versus control, green p < 0.05, yellow p < 0.01, orange p < 0.001. Figure S2. Mean ± SEM postprandial relative EPA, DHA, 17(S)-HDoHE, 17-keto-DHA in healthy subjects (N = 12) after ingestion of water (□), OGTT (◊), OLTT (△), OG + LTT (○). ANOVA: OGTT, OLTT, OL + GTT versus water: green p < 0.05, yellow p < 0.01, orange p < 0.001; water versus baseline; * p < 0.05, ** p < 0.01, *** p < 0.001.

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