Open Access Research article

Saliva sampling in global clinical studies: the impact of low sampling volume on performance of DNA in downstream genotyping experiments

David J Pulford1*, Michael Mosteller2, J David Briley2, Kelley W Johansson2 and Anita J Nelsen2

Author Affiliations

1 GlaxoSmithKline, Research & Development, Medicines Research Centre, Gunnels Wood Road, Stevenage, Herts SG1 2NY, UK

2 GlaxoSmithKline, Research & Development, Five Moore Drive, Research Triangle Park, NC 27709, USA

For all author emails, please log on.

BMC Medical Genomics 2013, 6:20  doi:10.1186/1755-8794-6-20

Published: 10 June 2013

Abstract

Background

The collection of viable DNA samples is an essential element of any genetics research programme. Biological samples for DNA purification are now routinely collected in many studies with a variety of sampling methods available. Initial observation in this study suggested a reduced genotyping success rate of some saliva derived DNA samples when compared to blood derived DNA samples prompting further investigation.

Methods

Genotyping success rate was investigated to assess the suitability of using saliva samples in future safety and efficacy pharmacogenetics experiments. The OrageneĀ® OG-300 DNA Self-Collection kit was used to collect and extract DNA from saliva from 1468 subjects enrolled in global clinical studies. Statistical analysis evaluated the impact of saliva sample volume of collection on the quality, yield, concentration and performance of saliva DNA in genotyping assays.

Results

Across 13 global clinical studies that utilized the OrageneĀ® OG-300 DNA Self-Collection kit there was variability in the volume of saliva sample collection with ~31% of participants providing 0.5 mL of saliva, rather than the recommended 2 mL. While the majority of saliva DNA samples provided high quality genotype data, collection of 0.5 mL volumes of saliva contributed to DNA samples being significantly less likely to pass genotyping quality control standards. Assessment of DNA sample characteristics that may influence genotyping outcomes indicated that saliva sample volume, DNA purity and turbidity were independently associated with sample genotype pass rate, but that saliva collection volume had the greatest effect.

Conclusion

When employing saliva sampling to obtain DNA, it is important to encourage all study participants to provide sufficient sample to minimize potential loss of data in downstream genotyping experiments.

Keywords:
Global study; Volume of saliva collection; DNA characteristics; Genotyping performance