Overview of the workflow followed in this work (left) and the proposed iterative Nonnegative Matrix Factorization (iNMF) clustering approach (right). (A) First, we clustered a dataset consisting of 62 CRC samples using NMF based on four selected pathways. (B) Then, we applied iNMF for stratifying the samples with an unbiased selection of probe sets and (C) matched CRC cell lines (CL) to the resulting clusters. (D) By overlaying matching pharmacology data, (E) we investigated the potential for generating testable hypotheses regarding response of cell lines in different clusters.
Schlicker et al. BMC Medical Genomics 2012 5:66 doi:10.1186/1755-8794-5-66