A series of three Zinc-Finger nuclease (ZFN 1–3) bound to FokI cleavage domain to form a dimer at a sequence specific site to introduce a double stranded break. The double stranded break introduced by the ZFNs will either repair itself with the Non-homologous end joining mechanism which results in the deletion of base pairs (1–300 bp) from the gene of interest. Alternatively a linearized construct can be introduced with the ZFNs and the repair will use the homology directed repair which inserts the transgene.
Walters et al. BMC Medical Genomics 2012 5:55 doi:10.1186/1755-8794-5-55