Additional file 3.

Additional materials and methods. Methods for plate manufacturing, PCR, calculation of log-expression ratios, PCR-efficiency, limits of detection, and limits of quantification are described (Additional files 8 and 9). (DOCX 213 kb)

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Bastien et al. BMC Medical Genomics 2012 5:44   doi:10.1186/1755-8794-5-44