Single agent dose-dependent cytotoxicity induced by cisplatin. Human bladder cancer cells UMUC9, UMUC14, SLT4, 253JBV, RT4, CLR2169, HT1197, 575A were seeded in 96 well plates and treatment with the indicated cisplatin concentration started 48 h after seeding and continued for 48 h. Cell viability was assessed with MTT-assay. Panel A: cell viability cisplatin dose response curve. Panel B: Cisplatin concentration killing 50% of the cells after 48 h relative to non-treated cells (GI50, Growth inhibition 50%). GI50 was estimated based on two independent experiments. Each drug concentration was tested in six individual wells.
Nordentoft et al. BMC Medical Genomics 2012 5:40 doi:10.1186/1755-8794-5-40