Email updates

Keep up to date with the latest news and content from BMC Medical Genomics and BioMed Central.

Open Access Highly Accessed Research article

MicroRNA profiling of a CD133+ spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line

Eun Ji Nam1, Maria Lee1, Ga Won Yim1, Jae Hoon Kim1, Sunghoon Kim1, Sang Wun Kim1 and Young Tae Kim12*

  • * Corresponding author: Young T Kim ytkchoi@yuhs.ac

  • † Equal contributors

Author Affiliations

1 Institute of Women’s Life Medical Science, Women’s Cancer Clinic, Department of Obstetrics and Gynecology, Yonsei University College of Medicine, Seoul, South Korea

2 Department of Obstetrics and Gynecology, Yonsei University College of Medicine, Seongsan-no 250, Seodaemun-gu, C.P.O. Box 8044, Seoul, South Korea, 120-752

For all author emails, please log on.

BMC Medical Genomics 2012, 5:18  doi:10.1186/1755-8794-5-18

Published: 29 May 2012

Abstract

Background

Cancer stem cells (CSCs) are thought to be a source of tumor recurrence due to their stem cell-like properties. MicroRNAs (miRNAs) regulate both normal stem cells and CSCs, and dysregulation of miRNAs has an important role in tumorigenesis. Cluster of differentiation (CD) 133+ and spheroid formation have been reported to be one of the main features of ovarian CSCs. Therefore, we determined the miRNA expression profile of a CD133+ spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line.

Methods

Initially, we confirmed the enrichment of the OVCAR3 CD133 subpopulation by evaluating in vitro anchorage-independent growth. After obtaining a subpopulation of CD133+ OVCAR3 cells with > 98% purity via cell sorting, miRNA microarray and real-time reverse transcription-polymerase chain reaction (RT-PCR) were performed to evaluate its miRNA profile.

Results

We found 37 differentially expressed miRNAs in the CD133+ spheroid-forming subpopulation of OVCAR3 cells, 34 of which were significantly up-regulated, including miR-205, miR-146a, miR-200a, miR-200b, and miR-3, and 3 of which were significantly down-regulated, including miR-1202 and miR-1181.

Conclusions

Our results indicate that dysregulation of miRNA may play a role in the stem cell-like properties of ovarian CSCs.

Keywords:
MicroRNA; Cancer stem cell; Ovarian cancer; CD133; OVCAR3; Chemoresistance