Experimental workflow and numbers of differentially expressed probes (DEPs) resulting from BMP4 or BMP7 treatment. (A) Seven breast cancer cell lines were cultured on 24-well plates, allowed to adhere for 24 h, and treated with the BMP ligand or vehicle for 30 min, 1 h, 3 h, 6 h, 12 h, and 24 h. Experiments were performed in triplicate, and collected cells were pooled. (B) The expression data from each cell line were individually filtered according to the following criteria: differential expression of at least 2-fold at a minimum of one time point. The number of DEPs per cell line is represented. (C) The expression data from individual time points of every cell line were filtered according to a 2-fold cutoff in expression change. The number of DEPs per time point is shown.
Rodriguez-Martinez et al. BMC Medical Genomics 2011 4:80 doi:10.1186/1755-8794-4-80