Validation of miR expression by quantitative real-time reverse transcription polymerase chain reaction (Q-RT-PCR). Q-RT-PCR analyses of miR-18a, miR-25, miR-1, and let-7g in 4 normal (shown in white) and 4 cancer samples (shown in black), confirming over-expression of miR-18a and miR-25 and under-expression of miR-1 and let-7g as observed in the microarray data of the cancer samples. The Student t-test P value between 4 normal and 4 cancer samples is shown for each miRNA. Fold change (FC) of -1 indicates a 50% decrease in RNU6-normalized expression of a given miRNA.
Kim et al. BMC Medical Genomics 2011 4:79 doi:10.1186/1755-8794-4-79