Table 1

Validation by qRT-PCR of FSHD dysregulation of gene expression

Microarray profiling

qRT-PCR


Gene

FSH/Ctl

FCb

p-value

FSH/Ctl

FCb

p-value

No. of

FSH

samples

No. of Ctl

samples


Myoblasts


PCOLCEa

5.9

4E-05

3.5

0.04

5

6

RUVBL2

2.2

5E-04

1.7

0.01

7

8

TGFB2

-2.8

4E-04

-1.9

0.02

9

8

EIF2C2

-1.6

0.007

-1.6

0.03

8

8

Myotubes


BGN

11.0

< 1E-06

16.8

0.04

8

9

DCN

6.4

6E-04

7.5

0.01

8

9

PCOLCE

10.2

< 1E-06

3.7

0.03

6

7

MYOM1

-8.9

2E-06

-2.9

0.05

4

4

SFRS9

3.5

< 1E-06

3.5

0.01

6

6

CAPG

3.0

0.002

3.2

0.02

8

9

IGFBP6

2.5

0.02

3.5

0.01

8

9

FRAS1

-3.02

0.001

-2.2

0.001

4

4


aFull gene names and primer-pairs are given in Additional File 5 TableS4

bFC, fold change in RNA signal was determined by exon microarray analysis or qRT-PCR

Tsumagari et al. BMC Medical Genomics 2011 4:67   doi:10.1186/1755-8794-4-67

Open Data