Creation of a minimal transcriptional regulatory network. Step 1: Differentially expressed genes (DEGs) are identified using SAM based selection. Step 2: Transcription Factors (TFs) and regulatory relationships (i.e. TF - TF) are identified among DEGs by using PAP. Step 3: A regulatory network is drawn by finding target genes regulated by TFs (i.e. TF - Target) from the DEG set using PAP. Step 4: A minimal network is generated by identifying targets regulated by more than one transcription factor.
Deshmukh et al. BMC Medical Genomics 2011 4:57 doi:10.1186/1755-8794-4-57