Open Access Research article

Detecting differential allelic expression using high-resolution melting curve analysis: application to the breast cancer susceptibility gene CHEK2

Tú Nguyen-Dumont1, Lars P Jordheim2, Jocelyne Michelon1, Nathalie Forey1, Sandrine McKay-Chopin1, Kathleen Cuningham Foundation Consortium for Research into Familial Aspects of Breast Cancer (kConFab)3, Olga Sinilnikova4, Florence Le Calvez-Kelm1, Melissa C Southey5, Sean V Tavtigian6 and Fabienne Lesueur1*

Author Affiliations

1 Genetic Cancer Susceptibility Group, IARC, 69372 Lyon, France

2 INSERM U590, Université Lyon 1, Lyon, France

3 Peter MacCallum Cancer Center, East Melbourne 2, VIC 3002, Australia

4 Unité Mixte de Génétique Constitutionnelle des Cancers Fréquents, Hospices Civils de Lyon, Centre Léon Bérard, 69373 Lyon, France

5 Department of Pathology, The University of Melbourne, VIC 3010, Australia

6 Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84112, USA

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BMC Medical Genomics 2011, 4:39  doi:10.1186/1755-8794-4-39

Published: 11 May 2011

Additional files

Additional file 1:

Mutation screening results for the 41 breast cancer samples enrolled in the DAE study. Additional table showing the mutation screening results for the 41 breast cancer samples enrolled in the DAE study.

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Additional file 2:

Primers and probes used in the DAE study on the CHEK2 gene. Additional table showing the primers and probes sequences used to perform the DAE study.

Format: DOC Size: 58KB Download file

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