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Interaction of a traditional Chinese Medicine (PHY906) and CPT-11 on the inflammatory process in the tumor microenvironment

Ena Wang1, Scott Bussom2, Jinguo Chen1, Courtney Quinn1, Davide Bedognetti13, Wing Lam2, Fulan Guan2, Zaoli Jiang2, Yichao Mark1, Yingdong Zhao4, David F Stroncek5, Jeffrey White6, Francesco M Marincola1* and Yung-Chi Cheng2*

Author Affiliations

1 Infectious Disease and Immunogenetics Section (IDIS), Department of Transfusion Medicine, Clinical Center and trans-NIH Center for Human Immunology (CHI), National Institutes of Health, Bethesda, Maryland, 20892, USA

2 Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut, 06520, USA

3 Department of Oncology, Biology and Genetics and Department of Internal Medicine, University of Genoa and National Cancer Research Institute of Genoa, Largo Rosanna Benzi 16132 Genoa, Italy

4 Division of Cancer Treatment and Diagnosis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20892, USA

5 Cell Therapy Section, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, Maryland, 20892, USA

6 Office of Cancer Complementary and Alternative Medicine, Division of Cancer Treatment and Diagnosis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20892, USA

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BMC Medical Genomics 2011, 4:38  doi:10.1186/1755-8794-4-38

Published: 11 May 2011

Additional files

Additional file 1:

Table S1. qPCR mouse primers used.

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Additional file 2:

Table S2. Summary of differentially expressed genes among treatment groups in different tissues.

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Additional file 3:

Figure S3. Stacked bar chart summarizing the 15 most affected canonical pathways according to IPA based on genes with annotated function differentially expressed (t-test cutoff p-value < 0.05, pt test p-value < 0.001) in tumor biopsies between PBS control group and the treatment groups: (a) PHY906 (b) CPT-11 (c) PHY906+CPT-11. Finally, differences between PHY906+CPT-11 compared to CPT-11 alone are shown in (d).

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Additional file 4:

Data S4. Cytotoxicity of PHY906 on HepG2 after three days exposure.

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