Dual exon skipping in myostatin and dystrophin for Duchenne muscular dystrophy
1 Center for Human and Clinical Genetics, Leiden University Medical Center, Postzone S4-P, PO Box 9600, Leiden, 2300RC, the Netherlands
2 Department of Molecular and Cell Biology, Leiden University Medical Center, Postzone S1-P, PO Box 9600, Leiden, 2300RC, the Netherlands
3 Institute for Molecular Cell Biology, University of Münster, Schlossplatz 5, Münster, D-48149, Germany
BMC Medical Genomics 2011, 4:36 doi:10.1186/1755-8794-4-36Published: 20 April 2011
Additional file 1:
Human primary control (KM109) myoblasts were differentiated for 7 days before transfection with 3 different MSTN AONs at 500 and 100 nM concentrations. All AONs were designed to target exon 2 of the myostatin gene. The sequences are listed in table 1. RNA was isolated 2 days post-transfection. cDNA was synthesized using random hexamer (N6) primers and subjected for PCR using primers in exon 1 and 3.
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