Open Access Highly Accessed Research article

Exploring the oral microbiota of children at various developmental stages of their dentition in the relation to their oral health

Wim Crielaard1, Egija Zaura1, Annemarie A Schuller2, Susan M Huse3, Roy C Montijn4 and Bart JF Keijser4*

Author Affiliations

1 Department of Experimental Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Free University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, the Netherlands

2 TNO Quality of Life, PO box 2215, 2301 CE Leiden, the Netherlands

3 Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA, USA

4 TNO Quality of Life, Business Unit Food and Biotechnology Innovations, Microbial Genomics Group, Utrechtseweg 48, 3704 HE Zeist, the Netherlands

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BMC Medical Genomics 2011, 4:22  doi:10.1186/1755-8794-4-22

Published: 4 March 2011

Additional files

Additional file 1:

Full list and relative abundance of higher taxa per group by health status and dentition stage, as obtained by 454 pyrosequencing. This file lists all 113 higher taxa (genera or more inclusive taxa when sequences could not be confidently classified to the genus level) and their relative abundance in saliva samples of children.

Format: PDF Size: 20KB Download file

This file can be viewed with: Adobe Acrobat Reader

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Additional file 2:

Rarefaction plot of the unique sequences in saliva samples of three oral health groups of children with early mixed dentition (healthy, treated or with caries). This is a rarefaction plot of all unique sequences by the number of sequences sampled in children with early mixed dentition by their oral health status.

Format: PPTX Size: 71KB Download file

Open Data

Additional file 3:

Microarray data. This is an Excel file with normalized microarray signal intensities for all samples used in this study.

Format: XLS Size: 509KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 4:

Full list of the microarray 16 S rDNA probes and their targets used in the microarray. This file lists all 16 S rDNA probes used in the microarray, probe sequences and their targets as identified by RDP blast search.

Format: PDF Size: 223KB Download file

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Additional file 5:

Distance matrix of saliva sample profiles from microarray data. This is a file with sample distance matrix calculated using Pearson correlation. The samples that belonged to siblings of one family are depicted with the same number. Samples that are numbered with 0, originated from children without siblings in the study group.

Format: PDF Size: 11KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data