Table 1

Clinical characteristics and outcomes of CLL patients

Patient's ID

Sex

Age (years)

Binet's stage

FISH Aberrations

Treatment

Matutes

sCD23 PVT

Apoptosis in vitro


B118R

Male

89

A

del(13q14) biallelic; del(11q22)monoallelic

yes

5

160

Resistant

G151R

Male

56

A

del(13q14)biallelic

yes

5

ND

Resistant

E147R

Male

70

A

del(13q14)biallelic; del(17p13)monoallelic

yes

4

ND

Resistant

B138R

Male

65

A

del(13q14)monoallelic

yes

5

ND

Resistant

RM1

Male

81

A

del(13q14)biallelic; del(17p13)monoallelic

yes

5

105

Resistant

RM2

Male

77

A

del(13q14)biallelic

yes

5

400

Resistant

RM3

Male

56

A

del(13q14)monoallelic

yes

4

102

Resistant

RF1

Female

74

A

del(13q14)biallelic; del(17p13)monoallelic

yes

5

443

Resistant

RF2

Female

67

A

del(13q14)monoallelic; del(17p13)monoallelic

yes

5

72

Resistant

RF3

Female

56

A

del(13q14)biallelic; del(17p13)monoallelic

no

5

ND

Resistant

U231R

Female

76

A

del(13q14)monoallelic

yes

5

ND

Resistant

G244S

Female

63

A

del(13q14)monoallelic; del(17p13)monoallelic

no

5

ND

Sensitive

B229S

Female

78

A

del(13q14)monoallelic

no

5

ND

Sensitive

S240S

Female

67

A

no

no

4

ND

Sensitive

SF1

Female

80

A

ND

no

4

70

Sensitive

SF2

Female

62

A

ND

no

4

28

Sensitive

SF3

Female

84

A

ND

no

5

97

Sensitive

SM1

Male

82

A

ND

no

4

52

Sensitive

SM2

Male

65

A

no

no

5

high

Sensitive

SM3

Male

74

A

del(13q14)monoallelic

no

4

low

Sensitive

L130S

Male

77

A

del(13q14)monoallelic

no

4

ND

Sensitive

S152S

Male

77

A

ND

no

4

458

Sensitive

B130S

Male

77

A

del(13q14)monoallelic

yes

4

93

Sensitive

B118S

Male

89

A

del(11q22) monoallelic

yes

4

160

Sensitive

M124S

Male

83

A

ND

no

5

ND

Sensitive


Apoptosis score was established 24 h after cell exposure to 10 Gy of γ-rays. Patients were considered sensitive (S) when the number of apoptotic cells was at least twofold higher in irradiated cells than in non-irradiated at 24 h of cell culture, or as resistant (R) when no difference was observed between irradiated and non-irradiated cells. Patients RM2 and B118S/R shifted from S to R status. Matutes scoring system was established to distinguish CLL from other lymphoproliferative disorders. This score is based on the immunophenotypic analysis of five markers: CD5+, CD23+, FMC7- and CD79b-, weak expression of monotypic κ or λ light chain. A value of 1 is assigned to a given marker with a level typical of CLL, and a total Matutes score at 4 or 5 indicates diagnosis of typical CLL. ND, not determined. Chromosomal aberrations were established by FISH using LSI ATM/LSI p53 and LSI D13S319/LSI 13q34/CEP12 probe sets (Abbott, France) which are complementary to 11q22.3, 17p13.1, 13q14.3, 13q34 and 12p11.1-q11 genome regions, respectively. To be taken into account, the aberration should be present in at least 5% of nuclei.

Marteau et al. BMC Medical Genomics 2010 3:53   doi:10.1186/1755-8794-3-53

Open Data