Research article
Molecular signature of response and potential pathways related to resistance to the HSP90 inhibitor, 17AAG, in breast cancer
1 Human Genetics Group, Spanish National Cancer Centre (CNIO), Madrid, Spain
2 Bioinformatics Unit. CNIO, Madrid, Spain
3 CIBERER, Centre for Biomedical Networking Research on Rare diseases, Madrid, Spain
BMC Medical Genomics 2010, 3:44 doi:10.1186/1755-8794-3-44
Published: 4 October 2010Additional files
Additional file 1:
Supplemental tables and figures. Supplemental Table S1: Oligonucleotides used for quantitative RT-PCR to validate expression of the selected from microarray study. Supplemental Table S2: List of the antibodies used for western blot. Supplemental Table S3: Top-ten pathways more associated to resistance to 17AAG. Supplemental Figure S1: Unsupervised clustering showed that samples mostly grouped by cell line. Some samples have technical replicates (marked with a star). Supplemental Figure S2: QT-PCR measurements of selected genes from the signature, confirmed the down regulation of five genes from the molecular signature (CYCLIN D1, JUNB, TMEM129, PLK3, NFKBIA) and up-regulation of UBE2C after treatment.
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