Open Access Research article

Molecular signature of response and potential pathways related to resistance to the HSP90 inhibitor, 17AAG, in breast cancer

Magdalena Zajac1, Gonzalo Gomez2, Javier Benitez13 and Beatriz Martínez-Delgado13*

Author Affiliations

1 Human Genetics Group, Spanish National Cancer Centre (CNIO), Madrid, Spain

2 Bioinformatics Unit. CNIO, Madrid, Spain

3 CIBERER, Centre for Biomedical Networking Research on Rare diseases, Madrid, Spain

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BMC Medical Genomics 2010, 3:44  doi:10.1186/1755-8794-3-44

Published: 4 October 2010

Additional files

Additional file 1:

Supplemental tables and figures. Supplemental Table S1: Oligonucleotides used for quantitative RT-PCR to validate expression of the selected from microarray study. Supplemental Table S2: List of the antibodies used for western blot. Supplemental Table S3: Top-ten pathways more associated to resistance to 17AAG. Supplemental Figure S1: Unsupervised clustering showed that samples mostly grouped by cell line. Some samples have technical replicates (marked with a star). Supplemental Figure S2: QT-PCR measurements of selected genes from the signature, confirmed the down regulation of five genes from the molecular signature (CYCLIN D1, JUNB, TMEM129, PLK3, NFKBIA) and up-regulation of UBE2C after treatment.

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