Promoter methylation and CpG content of decitabine-responsive genes in MCF-7 cell line. Of eighteen decitabine-induced genes from the MCF-7 breast cancer cell line, three are highly methylated, five have intermediate methylation levels, and ten have low levels of methylation. Highly methylated, decitabine-responsive genes have lower promoter CpG content. With increasing levels of methylation, CpG content of responsive genes increases. For extremely CpG-dense promoters, only those with low methylation levels are up-regulated. These observations are in accord with the melanoma data.
Rubinstein et al. BMC Medical Genomics 2010 3:4 doi:10.1186/1755-8794-3-4