Similarities in expression changes in trisomic APCs and astrocytoma cells were validated by RT-PCR analyses. A. Semi-quantitative RT-PCR analysis shows relative under expression of COL4A6, GABRA2, MGMT and TRPA1 (left panels), and over expression of HDAC9, CPXM1, DNMT3A, GUCY1A3 and STAT3 (right panels) transcripts in trisomic BG01V APCs and CCF-STTG1 astrocytoma cells with respect to diploid H9 APCs. 18S RNA was used as a control. B. Quantitative RT-PCR validation of relative changes in expression levels of the transcripts shown in panel A in diploid H9 APCs (blue bars), trisomic BG01V APCs (yellow bars) and astrocytoma cells (red bars).
Gopalakrishna-Pillai and Iverson BMC Medical Genomics 2010 3:12 doi:10.1186/1755-8794-3-12