Representative cases of LGALS4, CLU and ACS5 expression in matched normal mucosa (×100), and tumor tissue (×25). A-B-C: Normal sinusal mucosa immunostaining. (A-C): Weak and focal cytoplasmic staining of serous cells in a few seromucinous glands with LGALS4 and CLU. (A):Weak staining of respiratory epithelium with LGALS4. (B): Strong and diffuse immunostaining of serous cells with ACS5. D-E-F: Tumor immunostaining. (D): Poorly-differentiated "solid-type" component showing no immunoreactivity for LGALS4 while the "colonic-type" component is positive in a mixed ITAC (patient 5). (E): Example of ACS5 expression in a "colonic-type" ITAC. (F): No immunoreactivity for CLU in tumor samples (×100) except in one non-ITAC (Insert * (×25), Patient 11).
Tripodi et al. BMC Medical Genomics 2009 2:65 doi:10.1186/1755-8794-2-65