The relationship between IRF5 expression (measured by qPCR of the 3'-UTR of IRF5 in two regions of brain in cohort 3) and the IRF5 SNP rs10954213 genotype is shown. The ΔCt was calculated by subtracting from the Ct IRF5 the average Ct of two housekeeping genes (SNX16 and TSR1 in the anterior cingulate and SDHA and CAMK2A in the dorsolateral prefrontal cortex). A larger ΔCt indicates less expression of the target gene. The relationship between rs10954213 genotype and IRF5 probeset 3026432 expression was significant in these subjects in the DLPFC (F = 11.76; df = 1, 58;p = 8.11 × 10-5) and anterior cingulate cortex (F = 5.55; df = 1,58; p = 0.007) from cohort 2. The fold changes of expression in the anterior cingulate of subjects with the AG and GG genotypes compared to those with the AA genotypes were 0.52 and 0.50, respectively. The fold changes of expression in the anterior cingulate of subjects with the AG and GG genotypes compared to those with the AA genotypes were 0.33 and 0.38, respectively. Both regions showed a significant down-regulation in subjects with the AA genotype compared to the AG or GG genotype, consistent earlier reports of lower expression of the long 3'UTR in subjects carrying G alleles .
Martin et al. BMC Medical Genomics 2009 2:62 doi:10.1186/1755-8794-2-62