Correlation of array and qPCR results. (A) A scatterplot illustrating the association of raw miRNA array expression (log2) versus the negative CT value (Spearman's correlation 0.91) is used to assess the quantification capabilities of the array in a single sample. (B) All pairwise differences (log2) between the four cell lines were computed both within the array and qPCR data and plotted to assess the quantification capabilities of the array for relative differences between samples (Spearman's correlation 0.87). Included in the scatterplot are both the dotted y = x line as well as the solid regression fit line (y = -0.56 + 1.88x).
Cunningham et al. BMC Medical Genomics 2009 2:57 doi:10.1186/1755-8794-2-57