Table 3

Luminex-based multiplex cytokine assay showing the expression of chemokines and cytokines in the supernatants of HUVEC Vector and K13-ER cells with and without 4OHT treatment and following infection with KSHV.

Cytokines

Vector Mock treated

Vector + 4OHT

K13-ER-Mock treated

K13-ER+4OHT

KSHV Virus

Fold change K13-ER

Fold change KSHV

Fold change based on Microarray data


IL-1b

155.5

193.5

148

200

200

1.3

1.3

1.07

IL-2

20

23

20

22

21

1.1

1.05

1.01

IL-4

15

15

16

23

17

1.4

1.06

-2.9

IL-5

5

5

4

7

5

1.75

1.25

1.2

IL-6

1946.5

2038

1043.5

10910

11242

10.45

10.77

21.17

IL-8

6115

6682

4459.5

10336

10094.5

2.31

2.26

31.5

IL-10

1023

985

1003

1011.5

1038.5

1.00

1.03

3.3

IFN-γ

24.5

20

21

31.5

27

1.5

1.28

1.9

CSF2

13

15

10

1938

51

193.80

5.10

15.1

TNF-α

8

10

9

24

14

2.66

1.55

5.6

EOTAXIN

5

6

4

10

6

2.50

1.50

3.4

MCP-1

3217

2928

85

13178

10235.5

155.03

120.41

71.4

MIP-1α

37.5

31

24

117

105

4.87

4.37

3.6

MIP-1β

38

43

42.5

65

58

1.52

1.36

1.4

CCL5

327

345.5

305.5

11126.5

197

36.42

0.644

48.3

EGF

8798

8695

8711

8962

8765

1.02

1.00

16.5

VEGF

421

491

466.5

479

447

1.02

0.95

2.3

FGF-β

415

428

515

615.5

500

1.19

0.97

10.7

G-CSF

95

104

36

2125

604

59.02

16.77

3.9

HGF

134.5

143.5

148.5

155.5

264

1.04

1.77

3.2

CXCL10

498.5

485.5

70

10453

2259

149.32

32.27

47.7

IL-12p40

21

18.5

16.5

197

34

11.93

2.06

97

IL-13

7

8

7

13

8

1.85

1.14

1.4

IL-15

11

12

10

39

12

3.90

1.20

7.8

IL-17

118

139

130

129

132

0.99

1.01

-1.3

IFN-α

56

60

40

131

98

3.27

2.45

1.4

MIG

13

12

14

39

37.5

2.78

2.67

3.8

IL-1α

454.5

521.5

469

752

494.5

1.60

1.05

2.3

IL-7

58

50

47

197

181.5

4.19

3.86

2.1

IL-1Rα

30

31

28.5

90

59.5

3.15

2.08

3.8

IL-2R

12

16

15

21.5

18

1.43

1.20

2.6

GROα

13

13

14

110

17

7.85

1.21

7.7

MCP-2

26.5

25

23

37

23

1.60

1.00

4.4

MCP-3

12

14

10

23

372

2.30

37.20

2.3


Punj et al. BMC Medical Genomics 2009 2:50   doi:10.1186/1755-8794-2-50

Open Data